鼠巨细胞病毒感染小鼠感染性病毒滴度和基因表达的研究  

Study of the viral titer and genetic expression in mice with murine cytomegalovirus infection

作　　者：刘兴楼[1] 舒赛男[1] 王慧[1] 张慧娟[1] 李革[1] 方峰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,湖北武汉430030

出　　处：《临床儿科杂志》2011年第4期363-366,共4页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.30572345);教育部博士点基金(No.20090142110076)

摘　　要：目的观察鼠巨细胞病毒(MCMV)全身播散感染小鼠唾液腺的感染性病毒滴度,唾液腺、肝、脾和肾MCMV DNA及mRNA水平,以了解MCMV感染小鼠易感组织和器官内的病毒状况。方法 30只BALB/c幼龄小鼠腹腔接种MCMV(Smith株),在感染后1、3、7、14、28、60、75、90和120 d,各随机处死3只小鼠。噬斑法检测唾液腺感染性病毒滴度,实时定量PCR和逆转录PCR(RT-PCR)分别检测唾液腺、肝、脾和肾内MCMV DNA水平及mRNA表达。结果感染后7 d开始,噬斑法在唾液腺内检测到MCMV,14 d达到高峰,28 d以后不能再检测到;唾液腺、肝、脾和肾MCMV DNA在感染后1 d就可检测到,并持续到感染后120 d;唾液腺MCMV mRNA在感染后45 d不能检测到,而肝和肾MCMV mRNA在感染后28 d,脾MCMV mRNA在感染后14 d不能检测到。结论 MCMV感染的幼龄小鼠在感染28 d后不再排毒,在感染45 d后病毒不再复制,而在长达120 d的急慢性感染期内,病毒长期存在。Objectives To observe the murine cytomegalovirus（MCMV）infectious viral titer in salivary gland,the expression of MCMV DNA and mRNA in salivary gland,liver,spleen and kidney in MCMV disseminative infected mice,for understanding the MCMV status in susceptible tissues and organs in MCMV infected mice.Methods 30 BALB/c young mice were inoculated peritoneally with stocks of Smith strain MCMV and sacrificed 3 mice randomly at 1 d,3 d,7 d,14 d,28 d,45 d,60 d,75 d,90 d and 120 d post infection respectively.Plaque assay was used to determine MCMV infectious viral titer in salivary gland.Real-time quantitative PCR and Reversed transcript PCR were used to detect MCMV DNA and mRNA in salivary gland,liver,spleen and kidney.Results MCMV infectious viral titer was detected in salivary gland with plaque assay at 7d post infection,reached the peak at 14 d,and not detectable at 28 d.MCMV DNA was detected at 1d and remained detectable till 120 d post infection in salivary gland,liver,spleen and kidney.MCMV mRNA was not detected in salivary gland at 45 d post infection,liver and kidney at 28 d and spleen at 14 d.Conclusions In young mice,MCMV was no longer expelled at 28 d post infection,and no longer replicated at 45 d post infection,but the virus was always present during 120 d in acute and chronic phases of infection.

关 键 词：鼠巨细胞病毒 感染性病毒滴度 小鼠 

分 类 号：R363[医药卫生—病理学]