胶质瘤细胞系A172、U251、U87和SHG-44中HER2的表达及意义  

Expression of HER2 in glioma cell lines A172,U251,U87 and SHG-44 and its significance

作　　者：张雷鸣[1] 张剑宁[1] 杨双武[1] 董超[1] 方丹东[1] 李娟[1] 陈晓燕[1] 

机构地区：[1]第四军医大学西京脑科医院神经外科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2011年第2期151-153,共3页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(30872978;30801364)

摘　　要：目的探讨人表皮生长因子受体2(HER2)在人神经胶质瘤细胞系A172、U251、U87和SHG-44中的表达情况。方法采用W estern b lot和流式细胞术(FCM)于蛋白质水平检测HER2在四种人胶质瘤细胞系中的表达。结果 W estern b lot检测出HER2在人胶质瘤细胞系A172中的表达量最高,U251次之,U87和SHG-44表达量较低。FCM检测结果显示HER2在A172、U251、U87和SHG-44中的阳性率分别为36.5%、21.5%、3.3%和3.5%。结论人胶质瘤细胞系A172和U251中HER2的表达量较高,这两种细胞系可作为良好的细胞模型来研究HER2分子在胶质瘤中的作用机制以及以HER2为靶点的生物治疗等。Objective To investigate the expression of human epidermal growth factor receptor 2（HER2） in glioma cell lines A172,U251,U87 and SHG-44.Methods The expression of HER2 in human glioma cell lines A172,U251,U87 and SHG-44 was detected by Western blot and flow cytometry（FCM）.Results Western blot demonstrated that the expression of HER2 was the highest in A172 followed by U251,and lowest in the SHG-44 and U87.FCM analysis indicated that the positive expression rate of HER2 in A172,U251,U87 and SHG-44 cell lines were 36.5%,21.5%,3.3%,and 3.5%,respectively.Conclusion HER2 is highly-expressed in glioma cell lines A271 and U251,which can be used as an ideal cell model for the research of the role of HER2 in glioma and the specific therapies targeting HER2.

关 键 词：胶质瘤 HER2 细胞系 

分 类 号：R739.41[医药卫生—肿瘤]