骨碎补总黄酮对去卵巢骨质疏松模型大鼠血清中瘦素、白细胞介素6、前列腺素E_2及骨组织中β_2-肾上腺素受体的影响  

Effects of drynaria total flavonoids on serum levels of leptin,interleukin 6,prostaglandin E_2 and expression of boneβ_2-adrenergic receptor in ovariectomized osteoporosis rats

作　　者：孙影[1] 杨丽[1] 吕辰鹏[1] 方霁[2] 左娜[1] 沈家珍[2] 张荣华[1] 

机构地区：[1]暨南大学药学院中药学教研室,广东广州510632 [2]暨南大学医学院中医系,广东广州510632

出　　处：《中国病理生理杂志》2011年第4期755-758,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30772885)

摘　　要：目的:观察骨碎补总黄酮对去卵巢骨质疏松(OP)模型大鼠血清中瘦素(LEP)、白细胞介素6(IL-6)、前列腺素E_2(PGE_2)及骨组织中β_2-肾上腺素受体(ADRB2)的影响。方法:采用双侧卵巢切除法复制OP大鼠模型;造模12周后以双能X线法检测骨矿物质密度(BMD),确定OP模型是否复制成功;用药12周后应用酶联免疫法检测血清中LEP、IL-6和PGE_2的浓度,免疫组化法检测大鼠骨组织中ADRB2的表达。结果:造模12周后,模型组与假手术组相比,多个部位BMD显著下降(P<0.01),提示OP模型复制成功。用药12周后模型组LEP、IL-6和PGE_2较假手术组均有显著增高(P<0.05);骨碎补总黄酮组LEP和IL-6较模型组有明显降低(P<0.01),PGE_2无显著差异(P>0.05);模型组ADRB2表达与假手术组和给药组间比较有显著差异(P<0.05),假手术组和给药组间比较无显著差异(P>0.05)。结论:去卵巢OP大鼠血清LEP、IL-6、PGE_2和骨组织ADRB2表达升高;而骨碎补总黄酮能降低去卵巢OP大鼠血清中LEP、IL-6水平和骨组织ADRB2表达,抑制骨吸收,这可能是骨碎补总黄酮防治OP的作用机制之一。AIM：To investigate the effects of drynaria total flavonoids on serum levels of leptin（LEP）,interleukin 6（IL-6）,prostaglandin E_2（PGE_2）and the expression of boneβ_2-adrenergic receptor（ADRB2）in a rat model of ovariectomized osteoporosis（OP）.METHODS：The osteoporosis model was established by ovariectomy.Twelve weeks after modeling,bone mineral density（BMD）was determined by dual-energy X-ray absorptiometry to verify successful modeling.Enzyme-linked immunosorbent assay was applied to detect the concentrations of LEP,IL-6 and PGE_2 in serum. The expression of ADRB2 was determined by immunohistochemical technique.RESULTS：Compared with sham group,BMD of the rats in model group significantly decreased in multiple regions 12 weeks after modeling（P〈0.01）.The serum levels of LEP,IL-6 and PGE_2 in model group were significantly higher than those in sham group（P〈0.05）.The levels of LEP,IL-6 and PGE_2 in drynaria total flavonoids group were significandy lower than those in model group（P〈0.01）.No significant difference of PGE_2 between these 2 groups was observed.The ADRB2 expression in sham group and treatment group was significantly different from that in model group,and no significant difference between sham group and treatment group was found.CONCLUSION：The serum levels of LEP,IL-6 and PGE_2 and the expression of bone ADRB2 increased in OP rats.Drynaria total flavonoids reduce the production of LEP,IL-6 and the expression of ADRB2, and suppress the bone absorption,which may be one of the mechanisms in treating OP.

关 键 词：骨碎补总黄酮 瘦素 白细胞介素6 前列腺素E2 受体 肾上腺素能 β2 骨质疏松 

分 类 号：R[医药卫生]