大肠癌转移相关基因FMNL2的生物信息学分析  

Bioinformatic Analysis of the Gene FMNL2 in Clorectal Cancer Metastasis

作　　者：李余发[1] 肖法嫚[1] 梁莉[1] 丁彦青[1] 余力[1] 

机构地区：[1]南方医科大学珠江医院病理科,广州市510282

出　　处：《中国肿瘤临床》2011年第7期361-366,共6页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30400206;30870945);广东省自然科学基金(编号:915503101000016)资助~~

摘　　要：目的:研究各转移能力不同细胞系中的基因表达差异。方法:各转移能力不同的细胞系SW480、SW620、SW480/M5标本的mRNA与表达谱芯片杂交,获得表达差异基因。用生物信息学方法对基因FMNL2进行初步分析,以及用荧光定量PCR检测了转移能力不同的细胞系SW480、SW620、SW480/M5中FMNL2的表达。结果:表达谱芯片共找出了422条差异表达基因。用生物信息学方法分析了表达上调的基因FMNL2的结构、染色体定位、编码蛋白质的理化性质、亚细胞定位等信息。对FMNL2蛋白的多物种相似蛋白进行了系统进化分析,基本明确了该基因编码一种在胚胎和肿瘤细胞中高表达、进化中保守的核蛋白,以及证实了该基因在转移能力高的SW620和SW480/M5中比SW480中要高。结论:生物信息学分析表明,FMNL2是一个有研究价值的癌相关蛋白。这个蛋白可能参与正常的胚胎发育,但也与肿瘤转移能力有一定的关系。Objective： To explore the differences in gene expression in various cell lines of colorectal carcinoma （CRC） with different ability of metastasis. Methods： The mRNAs from the cell lines SW480, SW620 and SW480/M5 of different metastatic ability were labeled with biotin respectively, and were hybridized with expression profile microarray. The bioinformatic method was used to preliminarily analyze the gene FMNL2, and the expression of FMNL2 in the cell lines SW480, SW620 and SW480/M5 with different ability of metastasis was detected using fluorescent quantitation PCR. Results： A total of 422 differentially expressed genes were discovered by the expression profile microarray,. Bioinformatic analysis was conducted to explore the structure, chromosomal localization, physico-chemical property of the encoded protein, subcellular localization and fimctional domain of the upregulated gene FMNL2. The systematic evolution analysis of the similar proteins of FMNL2 was also performed. It basically confirmed a conservative nucleoprotein encoded by the gene in the high expression and evolution of the embryo and tumor cells. The real time quantitative PCR detection of FMNL2 gene found that the gene expression was 17.8 and 13.9 times higher in the SW620 and SW480/M5 cells than in the SW480 cells. Conclusion： Bioinformatic analysis indicates that FMNL2 is a tumor associated protein which may involve embryogenesis and tumor metastasis.

关 键 词：CDNA基因 表达谱芯片 大肠癌 生物信息学 FMNL2 

分 类 号：R735.34[医药卫生—肿瘤]