Genetic analysis and fine mapping of an enclosed panicle mutant esp2 in rice (Oryza sativa L.)  

作　　者：GUAN HuaZhong DUAN YuanLin LIU HuaQing CHEN ZhiWei ZHUO Ming ZHUANG LiJun QI WenMing PAN RunSen MAO DaMei ZHOU YuanChang WANG Feng WU WeiRen 

机构地区：[1]Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agricultural & Forestry University Fuzhou 350002, China [2]Fujian Provincial Key Laboratory of Marker-Assisted Breeding of Rice, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3]Fujian Agricultural Science Research Institute, Fuzhou 350002, China

出　　处：《Chinese Science Bulletin》2011年第14期1476-1480,共5页

基　　金：supported by the National Transgenic Projects of China (2009ZX08009-109B);Natural Science Foundation of Fujian Province (2010J01077);the National High Technology Research and Development Program of China (2006AA10Z128);New Century Excellent Talents in University of Fujian Province (KY0010057)

摘　　要：The phenomenon of panicle enclosure in rice is mainly caused by the shortening of uppermost internode.Elucidating the molecular mechanism of panicle enclosure will be helpful for solving the problem of panicle enclosure in male sterile lines and creating new germplasms in rice.We acquired a monogenic recessive enclosed panicle mutant,named as esp2 (enclosed shorter panicle 2),from the tissue culture progeny of indica rice cultivar Minghui-86.In the mutant,panicles were entirely enclosed by flag leaf sheaths and the uppermost internode was almost completely degenerated,but the other internodes did not have obvious changes in length.Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene,which could be steadily inherited and was not affected by genetic background.Apparently,ESP2 is a key gene for the development of uppermost internode in rice.Using an F 2 population of a cross between esp2 and a japonica rice cultivar Xiushui-13 as well as SSR and InDel markers,we fine mapped ESP2 to a 14-kb region on the end of the short arm of chromosome 1.According to the rice genome sequence annotation,only one intact gene exists in this region,namely,a putative phosphatidylserine synthase gene.Sequencing analysis on the mutant and the wild type indicated that this gene was inserted by a 5287-bp retrotransposon sequence.Hence,we took this gene as a candidate of ESP2.The results of this study will facilitate the cloning and functional analysis of ESP2 gene.The phenomenon of panicle enclosure in rice is mainly caused by the shortening of uppermost internode. Elucidating the molecular mechanism of panicle enclosure will be helpful for solving the problem of panicle enclosure in male sterile lines and creating new germplasms in rice. We acquired a monogenic recessive enclosed panicle mutant, named as esp2 （enclosed shorter panicle 2）, from the tissue culture progeny of indica rice cultivar Minghui-86. In the mutant, panicles were entirely enclosed by flag leaf sheaths and the uppermost internode was almost completely degenerated, but the other internodes did not have obvious changes in length. Genetic analysis indicated that the mutant phenotype was controlled by a recessive gene, which could be steadily inherited and was not affected by genetic background. Apparently, ESP2 is a key gene for the development of uppermost internode in rice. Using an F2 population of a cross between esp2 and a japonica rice cultivar Xiushui-13 as well as SSR and InDel markers, we fine mapped ESP2 to a 14-kb region on the end of the short arm of chromosome 1. According to the rice genome sequence annotation, only one intact gene exists in this region, namely, a putative phosphatidylserine synthase gene. Sequencing analysis on the mutant and the wild type indicated that this gene was inserted by a 5287-bp retrotransposon sequence. Hence, we took this gene as a candidate of ESP2. The results of this study will facilitate the cloning and functional analysis of ESP2 gene.

关 键 词：遗传分析 突变体 全封闭 水稻 精细定位 节间长度 磷脂酰丝氨酸 单基因 

分 类 号：S511[农业科学—作物学] TB652[一般工业技术—制冷工程]