Response surface optimization of microwave-assisted extraction for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii  

作　　者：Ying-Kun Liu E Yan Han-Ying Zhan Zhi-Qi Zhang 

机构地区：[1]Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry (Shaan.ri Normal University) Ministry of Education, Xi'an 710062, China [2]Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Forestry University,Lin'an 311300,China [3]Department of Chemistry,Qinghai,Normal University,Xining 810008,China

出　　处：《Journal of Pharmaceutical Analysis》2011年第1期13-19,共7页药物分析学报（英文版）

基　　金：the National Natural Science foundationof China(No.20875060) for financial support

摘　　要：Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol（1∶15,v/v）for 30 min,and then microwave irradiation for 11 min at a power of 600 W.Coupling the extraction process with HPLC-fluorescence presented good recovery,satisfactory precision,and good linear relation.Compared with a method from the Chinese Pharmacopoeia,the proposed method enables higher extraction efficiency and more accurate analytical results.It can be of potential value in quality assessment of Radix Puerariae thomsonii medicinal materials.Microwave-assisted extraction was optimized with response surface methodology for HPLC-fluorescence determination of puerarin and daidzein in Radix Puerariae thomsonii.The optimized extraction procedure was achieved by soaking the sample with 70% methanol（1∶15,v/v）for 30 min,and then microwave irradiation for 11 min at a power of 600 W.Coupling the extraction process with HPLC-fluorescence presented good recovery,satisfactory precision,and good linear relation.Compared with a method from the Chinese Pharmacopoeia,the proposed method enables higher extraction efficiency and more accurate analytical results.It can be of potential value in quality assessment of Radix Puerariae thomsonii medicinal materials.

关 键 词：microwave-assisted extraction（MAE） response surface methodology（RSM） PUERARIN DAIDZEIN HPLC-fluorescence detection Radix Puerariae thomsonii 

分 类 号：O657.72[理学—分析化学] TG142.45[理学—化学]