神经病理性疼痛大鼠海马NF-κB及TNF-α表达水平的变化  

Alteration of NF-kB and TNF-α mRNA and protein in hippocampus in the chronic constrictive injury model of rats

作　　者：于灵芝[1] 谭海波[1] 孙涛[2] 傅志俭[2] 

机构地区：[1]山东大学附属济南市中心医院疼痛科,济南250013 [2]山东大学附属济南市中心医院附属省立医院疼痛科,济南250013

出　　处：《中华行为医学与脑科学杂志》2011年第5期410-413,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金资助项目（30801072）;山东省自然科学基金资助项目（Q2007C14）

摘　　要：目的 观察慢性坐骨神经挤压性损伤（CCI）模型大鼠海马核因子-κB（NF-κB）及肿瘤坏死因子-α（TNF-α）表达的变化规律.方法 雄性Wistar大鼠76只,随机分为手术组（CCI组）和假手术组（Sham组）（每组n=38）,于CCI术前1天,术后1,4,7,14,21,28d各时间点测定机械痛阈及热痛阈后立即处死大鼠,取海马组织,采用荧光实时定量PCR方法测定NF-κB及TNF-α mRNA含量的变化,并与术后第7天采用免疫荧光的方法测定两种因子蛋白的表达.结果 与假手术组相比,CCI组大鼠手术侧机械痛阈及热痛阈明显降低.TNF-α及NF-κBmRNA表达水平于CCI术后1d和4 d开始增加,为术前的（2.079±0.104）倍,（1.640±0.064）倍,7 d达到高峰,为术前的（2.748±0.147）倍,（2.010 ± 0.096）倍,随后TNF-αmRNA表达水平迅速下降,而NF-κB mRNA于CCI后28 d仍为术前的（1.439±0.121）倍,维持于较高水平（P＜0.05）.术后第7天免疫荧光结果显示NF-κB及TNF-α在海马表达明显增加.结论 CCI致大鼠海马上调NF-κB及TNF-α的表达,可能参与神经病理性疼痛的调控过程.Objective To investigate the alteration of nuclear factor kappa B（ NF-κB） and tumor necrosis factor-a （TNF-α） mRNA and protein in hippocampus in chronic constrictive injury （CCI） model of rats. Methods Seventy-six male Wistar rats were randomly divided into 2 groups （ n = 38）： the CCI group which received the chronic constriction injury and the sham group which received the sham operation as control. The mechanical and thermal nociceptive thresholds were assessed with paw withdrawal latency （PWL） to von Frey filaments and radiant heat at 1d before and ld,4d,7d,14d and 28d after CCI operation. Five animals were sacrificed at each time point for real-time polymerase chain reaction （real-time PCR） and another three animals sacrificed at 7d postoperation for immunofluorescence histochemical staining. Results The thresholds to mechanical and thermal stimuli decreased obviously after operation in CCI group. The expressions of TNF-α and NF-κB mRNA began to increase at ld（ （2.079 ±0. 104）times and 4d（ （ 1.640 ± 0.064） times） after operation and reached the peak at 7d （（2.748 ±0.147）times, （2.010 ±0.096）times） ,then the expressions of TNF-a mRNA began to decrease,while the expressions of NF-kB mRNA maintained at a high level throughout the experiment. The result of immunofluorescence histochemical staining revealed that NF-kB and TNF-α protein expressions at 7 day increased significantly on the hippocampus,which was consisted with NF-κB and TNF-a mRNA levels. Conclusion The activation NF-κB and TNF-α in hippocampus may be involved in the procession of neuropathic pain.

关 键 词：神经病理性疼痛 海马 海马核因子-κb 肿瘤坏死因子-Α 

分 类 号：R741[医药卫生—神经病学与精神病学]