高良姜油抗实验性胃溃疡作用及其机制研究  

Research on alpinia officinarum hance oil against experimental gastrelcosis and action mechanism

作　　者：王海燕[1,2] 刘亚明[3] 牛欣[1] 冯前进[3] 李海燕[1] 郭建友[4] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]湖北中医药大学基础医学院,湖北武汉430061 [3]山西中医学院方剂教研室,山西太原030024 [4]中国科学院心理研究所心理健康重点实验室,北京100101

出　　处：《中国中西医结合消化杂志》2011年第2期71-74,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：科技部国家"十五"攻关计划项目(2004BA721A46)

摘　　要：[目的]研究高良姜油抗胃溃疡(GU)的作用及其机制。[方法]ICR小鼠随机分成7组,分别为:正常对照组,模型组,奥美拉唑组(0.014 g/kg),良附丸组(2 g/kg),高良姜油高、中、低剂量(8、42、ml/kg)组,预防给药6d后,采用利血平致GU小鼠模型,收集小鼠胃液,测定胃液量、总酸度、胃蛋白酶活性及血清一氧化氮(NO)、超氧化物歧化酶(SOD)及丙二醛(MDA)水平。[结果]与模型组相比,高良姜油高、中剂量组,良附丸组,奥美拉唑组均能抑制利血平致GU小鼠模型胃液量、总酸度及胃蛋白酶活性,且能增加血清NO水平和SOD活性,降低MDA水平;但中剂量组胃蛋白酶活性变化无统计学意义。[结论]高良姜油抗GU作用机制可能与其抑酸、抗氧化、增强保护因子有关;高良姜油高剂量抗氧化与增加血清NO水平的作用与良附丸相当。[Objective]To study the protective effect of alpinia officinarum hance oil（AOHO）on the experimental gastrelcosis.[Methods]ICR mice were randomly divided into 7 groups：control group,model group,Omeprazole group（0.014g·kg-1）,Liangfu Pill group（2g·kg-1）,AOHO high dosage group,AOHO middle dosage group,AOHO low dosage group（8,4,2mL·kg-1）.After 6-day treatment for prevention,the mice were given reserpine to induce gastrelcosis model.Thereafter,gastric juice was collected from all mice in each group.The gastric juice volume,total acidity and activity of pepsin were measured.Nitric oxide（NO）content,superoxide dismutase（SOD）activity and malondialdehyde（MDA）content in mice serum were determined.[Results]Compared with those in the model group,the gastric juice volume and total acidity were lowered,the activity of pepsin was inhibited respectively,except for those in the AOHO low dosage group.Furthermore,NO content and SOD activity increased and MDA content decreased.[Conclusion]AOHO is effective in treating gastrelcosis by inhibiting acidity,improving antioxidant capacity,increaseing protective factors.

关 键 词：胃溃疡 高良姜油 一氧化氮 超氧化物歧化酶 丙二醛 

分 类 号：R573.1[医药卫生—消化系统] R282.71[医药卫生—内科学]