2008—2009年沈阳市部分季节性流感病毒H1N1亚型HA1基因特点  

Characteristics of H1N1 Hypotype HA1 Gene at The Season of Influenza Pandemic

作　　者：蔡特[1] 王冰[1] 高慧卉[1] 

机构地区：[1]辽宁省大连市西岗区疾病预防控制中心检验科,116011

出　　处：《职业与健康》2011年第10期1109-1110,共2页Occupation and Health

摘　　要：目的了解沈阳市2008—2009年流行性感冒（流感）流行季节H1N1亚型的HA1基因变异特征。方法利用沈阳市流感病毒H1N1亚型分离株,采用一步RT-PCR（反转录PCR）方法扩增HA1基因,测序,推到氨基酸序列,构建种系进化树。结果 2008—2009年共采集流感样病例咽拭子样本177份,共分离到流感病毒29株,分离率为16.4%。其中H1N1亚型21株。测定了10株H1N1亚型流感病毒分离株的HA1序列,与WHO推荐疫苗株A/B risbane/59/2007比对,同源性为94.2%~97.0%,氨基酸位点99、202、208、211、230发生了突变。结论沈阳市2008—2009年流感病毒发生了一定的变异,造成了该流行季节的H1N1亚型流感病毒流行。[Objective]To indentify the variability of H1N1 hypotype HA1 gene at the season of influenza pandemic between 2008 and 2009 in Shenyang City.[Methods]The H1N1 hypotype isolation of influenza virus from Shenyang was sequenced the amplification of HA1 gene by one-step method of PT-PCR,then deduced the amino acid sequence and constructed the tree of phyletic evolution.[Results]Of 177 throat swab samples of influenza-like illness,29 strains of influenza virus（16.4%） were isolated,21 were H1N1 Hypotype.Assayed HA1 sequence10 from the 21 strains,compared with vaccine strain of A/Brisbane/59/2007 recommended by WHO,the homology could reach 94.2%~97.0%,the mutation of amino acid appeared at the site of 99,202,208,211 and 230.[Conclusion]A certain variation of influenza virus occurred in Shenyang between 2008 and 2009,and it caused the influenza pandemic of H1N1 hypotype in that season.

关 键 词：流感病毒 血凝素基因重链 

分 类 号：R181.3[医药卫生—流行病学]