七氟醚预处理对大鼠急性肾缺血再灌注损伤肾功能的影响  

Effects of sevoflurane preconditioning on renal ischemiareperfusion injury renal function in rats

作　　者：顾福萍[1] 何焕钟[2] 顾红光[1] 顾栋桦[2] 沈洪[1] 

机构地区：[1]浙江省湖州师范学院医学院,湖州313000 [2]浙江省湖州市中心医院

出　　处：《陕西医学杂志》2011年第5期517-519,共3页Shaanxi Medical Journal

基　　金：湖州市自然科学资金项目(No2009YZ05)

摘　　要：目的:探讨七氟醚预处理对大鼠急性肾缺血再灌注损伤肾功能的影响。方法:将SD雄性大鼠72只随机分为假手术组(C组)、缺血再灌注组(I/R组)和七氟醚预处理组(S组),每组24只,后两组根据再灌注时间分为再灌注后4、12、24 h 3个亚组,每个亚组8只。实验结束检测血BUN、Cr,观察肾组织的病理变化,对肾小管损害进行评分,并进行比较。结果:S组和I/R组BUN、Cr均较C组明显升高(P<0.05),再灌注后同时相点比较,12h、24h S组BUN、Cr均明显低于I/R组,4h两组差异不显著;肾小管Paller氏评分,与C组相比,I/R、S组均高于C组(P<0.05),S组各时相点评分较I/R组降低(P<0.05)。结论:七氟醚预处理对大鼠急性肾缺血再灌注损伤有保护作用。Objective： To investigate the effects of sevoflurane pretreatment on renal ischemia-reperfusion injury and renal function in rats.Methods： Seventy-two adult male Sprague-Dawley rats were randomly divided into three groups（n=24）.①C group：Performed a similar operation without occlusion of renal blood flow.②I/R group：Performed the operation of renal ischemiareperfusion（I/R）.③S group：inhalation of 2.2%（1MAC） sevoflurane for 60 minute were performed before I/R.After 45 minutes of ischemia and 4,12,24h of reperfusion,serum BUN,Cr levels were measured.The kidney slices were observed under microscope and renal pathological changes were recorded,the tubular damage was scored.Results： after reperfusion,the serum BUN,Cr level in S group and I/R group were significantly higher than those in C group（P0.05）and which went up with the increased reperfusion time in I/R group;the serum BUN,Cr levels S group were significantly lower than those in I/R group after reperfusion 12h,24h（P0.05）.The Paller＇s score for actue renal tubular injury in I/R group increased markly compared with that in C group（P0.05）,which in S group decreased markly compared with that in I/R group（P0.05）.Conclusion： Pretreatment with 2.2% sevoflurane for 60 minutes can provide significant protection against renal ischemia-reperfusion injury.

关 键 词：再灌注损伤/病理生理学 再灌注损伤/药物疗法 麻醉药 吸入/治疗应用 醚类/治疗应用 血尿素氮/代谢 动物 实验 大鼠 

分 类 号：R361[医药卫生—病理学]