喉癌患者外周血Cathepsin B、L及Stefin A水平检测的意义  

Significance of measuring cathepsin B and L as well as stefin A levels in peripheral blood of patients with laryngeal carcinoma

作　　者：陈立伟[1] 刘明波[2] 王嘉陵[2] 赵建东[2] 田军[1] 

机构地区：[1]潍坊医学院耳鼻咽喉头颈外科,潍坊261053 [2]解放军总医院耳鼻咽喉头颈外科,北京100853

出　　处：《军医进修学院学报》2011年第6期620-621,628,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30872853)~~

摘　　要：目的探讨喉癌患者外周血组织蛋白酶B(Cathepsin B)、组织蛋白酶L(Cathepsin L)及内源性抑制剂(Stefin A)表达水平的变化与临床诊断和预后的关系。方法收集我院确诊的喉癌患者及正常对照者血清,酶联免疫吸附测定法(Elisa)检测60例喉癌患者及30位正常对照者血清中Cathepsin B、L及Stefin A水平。结果喉癌患者血清中CathepsinB、L水平显著高于正常对照者(P<0.01),二者表达水平呈显著正相关(r=0.434 1,P<0.05);Stefin A水平显著低于正常对照者(P<0.01),但与Cathepsin B、L无显著相关性。结论喉癌患者血清中存在高水平的Cathepsin B、L及低水平的Stefin A,提示检测血清中Cathepsin B、L及Stefin A含量可协助诊断及判断预后。Objective To study the relation between expression levels of cathepsin B and L as well as stefin A in peripheral blood of patients with laryngeal carcinoma and its diagnosis and prognosis.Methods Cathepsin B and L as well as stefin A levels in serum of 60 laryngeal carcinoma patients and 30 normal controls were measured by enzyme linked immunosorbent assay.Results The levels of cathpsin B and L were significantly higher in serum of laryngeal carcinoma patients than in normal controls（P〈0.01）.The expression levels of cathpsin B and L were positively correlated（r=0.4341,P〈0.05）.The stefin A level was significantly lower in serum of laryngeal carcinoma patients than in normal controls（P〈0.01） but not correlated with the levels of cathpsin B and L.Conclusion High cathpsin B and L level of and low stefin A level in serum of laryngeal carcinoma patients contribute to the diagnosis of laryngeal carcinoma and judgment of its prognosis.

关 键 词：CATHEPSIN-B CATHEPSIN-L Stefin-A 喉癌 

分 类 号：R73[医药卫生—肿瘤]