淫羊藿提取物IC163对K562细胞的诱导分化作用观察  

Differentiation-inducing effects of IC163 on K562 cells

作　　者：朱剑锋[1] 吴正东[1] 孟坤 张广森[3] 

机构地区：[1]泰州市人民医院血液科,江苏泰州225300 [2]北京珅奥基医药科技有限公司 [3]中南大学湘雅二医院血液科

出　　处：《临床血液学杂志》2011年第3期300-302,共3页Journal of Clinical Hematology

摘　　要：目的:观察淫羊藿提取物IC163对K562细胞是否有分化诱导作用。方法:用不同浓度IC163作用于K562细胞6d,通过W-G染色观察细胞形态学变化、细胞内血红蛋白测定、细胞内血红蛋白联苯胺染色和流式细胞术检测膜CD71和CD235a分化抗原4个方面来评价IC163对K562细胞的诱导分化作用。结果:8μmol/L的IC163可明显上调K562细胞膜CD71和CD235a分化抗原的表达,升高细胞内Hb含量,在形态上有趋向红系分化的改变。结论:IC163有诱导K562细胞分化的作用。Objective：To determine the differentiation-inducing potentiality of IC163 on K562 cells.Method：Differentiation induction experiment was performed by culturing K562 cells with the presence of IC163（0-64 μmol/L） for 6 days.The changes of morphology,cytochemisrty and cytophyletic characteristic markers（CD71 and CD235a） of K562 cells were observed before and after the treatment with IC163,by the methods of wright＇s-Giemsa staining,benzidine staining and flowcytometry respectivly.Result：K562 cells treated with 8 μmol/L IC163 were found differentiated and morphologically changed to polychromatic normoblasts and orthrochromatic normoblasts.Treatment of K562 cells with IC163 increased hemoglobin content,benzidine-positive cells from（4.03±1.46）%（0 μmol/L） to（13.39±2.49）%（8 μmol/L） and up-regulated the expressions of CD71 and CD235a.Conclusion：IC163 can induce K562 cells differentiation.

关 键 词：白血病 粒细胞性 慢性 IC163 K562细胞 分化 

分 类 号：R733.72[医药卫生—肿瘤]