苦瓜根提取物对α-葡萄糖苷酶抑制作用研究  

Study on theα-Glucosidase Inhibitory Activities of Different Extract Parts from the Bitter Gourd Root

作　　者：陈林[1,2] 冯天艳[3] 谢瑜[3] 何秀丽[2] 

机构地区：[1]湖北中医药大学中药配伍与中药复方教育部重点实验室,武汉430065 [2]湖北医药学院附属太和医院 [3]三峡大学天然产物开发与利用湖北省重点实验室

出　　处：《中国药师》2011年第5期634-636,共3页China Pharmacist

基　　金：中药资源与中药复方省部共建教育部重点实验室开放基金

摘　　要：目的:通过α-葡萄糖苷酶活性抑制实验研究苦瓜根提取物对α-葡萄糖苷酶的抑制活性,筛选其活性部位。方法:采用乙醇回流提取,有机溶剂萃取,得到石油醚、乙酸乙酯、正丁醇等不同萃取部位,检测醇提物和各萃取部位对α-葡萄糖苷酶活性的抑制作用。结果:苦瓜根醇提物不同萃取部位对α-葡萄糖苷酶均有一定的抑制作用,与空白对照组对比,差异具有显著性(P<0.01);其中水部位的抑制作用最强,当浓度为100μg·ml^(-1)时,抑制率达到61%。结论:苦瓜根醇提物不同萃取部位对α-葡萄糖苷酶均有抑制作用,其中水部位的抑制作用最强。Objective： To study the α-glucosidase inhibitory activities of different extract parts from root of the bitter gourd root and screen the active part. Method： Organic solvent extract after ethanol circumfluence to obtain petroleum benzine, acetidin, n-butanol and water extraction parts. PNPG colorimetry was used to detect the α-glucosidase inhibiting activity of ethanol extract and the other parts. Result： All of the extract parts of root of the bitter gourd root had different inhibiting effect to α-glucosidase. There were significant differences between the extract parts and control group （P 〈 0. 01 ）. Water part had the strongest inhibitory activity, when the concentration was 100 μg·ml^-1 ,the inhibition rate got to 61%. Conclusion： Different extract parts of the bitter gourd root have inhibiting effect on α-glucosidase and the water part have the strongest effect.

关 键 词：苦瓜根 萃取 Α-葡萄糖苷酶 抑制作用 

分 类 号：R285.5[医药卫生—中药学]