阿魏酸对bFGF诱导PC12细胞分化的影响  

Effects of Ferulic Acid on bFGF-treated PC12 Cells

作　　者：林炼峰[1] 肖飞[1] 赖永长[1] 苏锯年[1] 罗焕敏[1,2] 

机构地区：[1]暨南大学医学院药理学系,广东广州510632 [2]暨南大学-香港大学脑功能与健康联合实验室,广东广州510632

出　　处：《中药材》2011年第4期572-575,共4页Journal of Chinese Medicinal Materials

基　　金：国家"211工程"建设经费资助

摘　　要：目的:探讨阿魏酸在低浓度碱性成纤维生长因子(bFGF)诱导大鼠肾上腺髓质嗜铬瘤细胞(PC12细胞)分化的作用及其初步机制。方法:在0 ng/mL或1 ng/mL bFGF条件下培养PC12细胞,以PC12细胞的突起长度及诱导率确定药效。结果:阿魏酸在无bFGF的情况下不能诱导PC12分化,但在低浓度bFGF(1 ng/mL)情况下,与bFGF(1 ng/mL)对照组比较可明显诱导PC12细胞分化(P<0.01),这种作用能被有丝分裂原激活的蛋白激酶(MAPK)特异性抑制剂PD98059所抑制。结论:阿魏酸可增强bFGF诱导PC12细胞分化,其机制可能与MAPK信号通路有关。Objective：To explore the effect and mechanism of ferulic acid on differentiation in bFGF-treated PC12 cells.Methods：The length of neurite outgrowth and the percentage of PC12 cells induced in the presence of 0 ng/mL or 1 ng/mL bFGF were assayed.Results：Compared with that of control group,ferulic acid could enhance the differentiation effect of bFGF（1 ng/mL） in PC12 cells（P0.01） and the enhancing effect could be blocked by the specific MAPK kinase inhibitor,PD98059.Conclusion：Ferulic acid potentiates neurite outgrowth in bFGF-treated PC12 cells by MAPK-dependent signaling pathway.

关 键 词：阿魏酸 PC12细胞 突起生长 BFGF 

分 类 号：R285.5[医药卫生—中药学]