p16、COX-2在喉癌组织中的表达及相关性研究  

Expression of p16 and cyclooxygenase-2 in larynx carcinoma tissues and their correlation

作　　者：李俊义[1,2] 徐华林[1,2] 徐峰[1,2] 

机构地区：[1]山东省莱芜市人民医院 [2]泰山医学院附属莱芜医院耳鼻咽喉科,271100

出　　处：《中国中西医结合耳鼻咽喉科杂志》2011年第2期72-74,共3页Chinese Journal of Otorhinolaryngology in Integrative Medicine

摘　　要：目的研究喉癌组织中抑癌基因p16及环氧化酶-2(COX-2)的表达,探讨p16、COX-2在喉癌发生发展中的作用及两者之间的关系。方法采用免疫组织化学方法检测50例喉癌组织中p16、Cox-2的表达情况。结果 50例喉癌标本中喉癌中p16蛋白表达的阳性率为56.0%,正常喉黏膜中阳性表达率为80.0%,p16蛋白在喉鳞癌组织中的阳性表达率明显低于正常喉黏膜组织(P<0.05),有统计学意义。随着临床分期的进展及组织学分化程度的降低,p16蛋白表达进一步降低。COX-2在喉癌组织中高表达,阳性表达率为62.0%,正常组织阳性率为24%,患者的不同临床分期的表达差异有统计学意义(P<0.05)。p16与COX-2之间存在负相关性。结论两者的联合检测对估计喉癌的恶性度、判断预后有一定意义。Objective To investigate the expression of p16 and cyclooxygenase-2 （Cox-2） in laryngeal squamous carcinoma and analyze the relationship p16 and Cox-2. Methods 50 cases of laryngeal squamous carcinoma were detected by using immunohistochemistry. Results The positive rate ofpl6 expression in laryngeal squamous carcinoma was 56.0%, but it was 80.0% in the normal laryngeal mucosa. The positive rate of pl 6 in laryngeal squamous carcinoma were significantly lower than that in the normal tissues （P〈 0. 05）. Furthermore, p16 expression showed a declined tendency in the positive rate in cases with the aggravation of clinical stag and cell differentiation. Overexpression of Cox-2 was found in the laryngeal squamous carcinoma specimens. The positive rate of Cox-2 was 62.0%, and it was related to the clinical stage and the histological grade, had a negative correlation with p16. Conclusions Combinative detection ofpl6 and cyclooxygenase-2 could have significance in predicting the biological aggressiveness and clinical outcome of laryngeal carcinoma.

关 键 词：喉癌 P16基因蛋白 环氧化酶-2 

分 类 号：R739.65[医药卫生—肿瘤]