伴2型糖尿病慢性牙周炎患者TNF-α基因携带频率的研究  

A study of frequency of TNF alpha gene with type 2 diabetes mellitus with chronic periodontitis

作　　者：刘博[1] 俞宁[1] 谭丽思[1] 刘静波[1] 郭艳[2] 潘亚萍[1] 

机构地区：[1]中国医科大学口腔医学院牙周科,辽宁沈阳110002 [2]中国医科大学口腔医学院中心实验室,辽宁沈阳110002

出　　处：《上海口腔医学》2011年第2期169-173,共5页Shanghai Journal of Stomatology

基　　金："十一五"国家科技支撑项目(2007BAl18B02)~~

摘　　要：目的:通过对伴2型糖尿病慢性牙周炎患者、不伴全身系统性疾病的慢性牙周炎患者以及健康对照组中TNF-α基因携带频率的分析,探讨病例组和对照组在该基因携带频率上的差异,并比较各组牙周临床指标和易感等位基因的关系。方法:采用牙周探针,对112例伴2型糖尿病慢性牙周炎患者(DM组)、99例单纯慢性牙周炎患者(CP组)以及健康对照组进行牙周临床指标检查和TNF-α-308基因型(TNF1/2)检测。采用SPSS13.0软件包对数据进行χ2检验和方差分析。结果:在DM组和轻中度CP组之间,轻中度DM组和重度CP组之间,重度DM组和轻中度CP组之间,重度DM组和重度CP组之间,TNF2的阳性基因型分布均有统计学差异(P<0.05)。携带等位基因TNF2的DM组和CP组的牙周探诊深度、临床附着丧失均分别显著高于只携带等位基因TNF1的DM组和CP组患者(P<0.05)。结论:携带TNF-α-308等位基因TNF2可能会增加人群牙周炎的易感性,并且在2型糖尿病和牙周炎协同作用过程中具有重要作用。PURPOSE： To detect the frequency of TNF alpha gene in patients of type 2 diabetes mellitus with chronic periodontitis,periodontitis without any systemic diseases and healthy controls.METHODS： The case series were consisted of 112 patients with moderate,severe type 2 diabetes mellitus with chronic periodontitis,99 patients with moderate,severe periodontitis without any systemic disease,50 age-and gender-matched subjects with healthy periodontal conditions were enrolled.Clinical parameters were measured and recorded including probing depth（PD）,clinical attachment loss（CAL）,bleeding index（BI）,and tooth movement（TM）.The polymorphism of TNF-α-308 genotype（TNF1/2）was examined after electrophoresis on agarose gel and ethidium bromide staining.The difference between the case and healthy groups was analysed by Chi-square test,the difference in clinical index among groups which had different allele was analyzed for ANOVA with SPSS13.0 software package.RESULTS： We divided DM and CP groups into moderate and severe groups.There were significant difference between severe DM group and severe,moderate CP group,moderate DM group and chronic periodontitis of severe、moderate group.The probing depth and clinical attachment loss of the patients who took TNF-α-308 allele Ⅱ were significantly higher than the patients who took TNF-α-308 allele Ⅰ in DM and CP group.CONCLUSIONS： TNF-α-308 alleleⅡmight increase the susceptivity of periodontitis in population.TNF-α-308 allele Ⅱmay play an important role in synergistic reaction of periodontitis and type 2 diabetes.

关 键 词：基因 2型糖尿病 慢性牙周炎 频率 

分 类 号：R781.4[医药卫生—口腔医学]