三氯醋酸-丙酮沉淀法用于骨组织蛋白的提取  

Application of Trichloroacetic Acid-Acetone Precipitation Method for Protein Extraction in Bone Tissue

作　　者：崔永锋[1] 杨贞[2] 朱宝华[1] 肖鲁伟[2] 童培建[2] 

机构地区：[1]杭州市萧山区第一人民医院骨科,杭州311200 [2]浙江中医药大学骨伤科研究所,杭州310053

出　　处：《中国医学科学院学报》2011年第2期210-213,共4页Acta Academiae Medicinae Sinicae

基　　金：中国博士后科学基金(20080431334)~~

摘　　要：目的探讨三氯醋酸-丙酮沉淀法提取骨组织蛋白的可行性和效率。方法采用盐酸脱钙法和三氯醋酸-丙酮沉淀法,分别用于骨组织蛋白的提取,并对两种方法的提取效率进行对比。结果三氯醋酸-丙酮沉淀法可获得较高的骨蛋白提取率,与盐酸脱钙法相比,分子条带分布范围相近,且不受骨髓造血组织影响。结论三氯醋酸-丙酮沉淀法可以用于骨组织蛋白质组的研究。Objective To explore the feasibility and efficiency of extracting protein from bone tissue using trichloroacetic acid（TCA）-acetone precipitation method.Methods Hydrochloric acid（HCL） decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction.The efficiencies of these two methods were compared.Results TCA-acetone precipitation method had significantly higher extraction efficiency.Compared with HCL decalcification method,it had less pollution from bone marrow hematopoietic tissue.Protein band distribution was similar between these two methods.Conclusion TCA-acetone precipitation method is useful for bone proteomics research.

关 键 词：三氯醋酸-丙酮沉淀法 骨组织 蛋白提取 

分 类 号：Q-34[生物学]