正交试验优选广枣提取工艺  

Optimization of Extraction Technology of Fructus Choerospondiatis by Orthogonal Test

作　　者：黎瑞汝[1] 苏智斌[1] 陈曦[1] 王淑美[1] 梁生旺[1] 

机构地区：[1]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2011年第10期47-49,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选广枣药材的最佳提取工艺。方法:通过正交试验考察乙醇体积分数、加乙醇量、提取时间、提取次数4个因素,采用反相高效液相法测定没食子酸含量,紫外-分光光度法测定总黄酮。结果:最佳的提取工艺是加8倍量70%乙醇回流提取2次,每次60 min。结论:该提取工艺经济、可行、可控。Objective：To optimize the extraction technology for traditional Mongolian medicine Fructus Choerospondiatis.Method：The extraction technology was optimized by orthogonal design with concentration of alcohol,amount of alcohol,extraction time and extraction times as factors.A RP-HPLC.Result：was established for determination of gallic acid;An UV-Spectrophotometer method was adopted to determine the content of total flavonoids.Result：The optimum extracting condition was as follows：adding eight times amount of 70% alcohol and refluxing and extracting for 1h（totally extracting for 2 times）.Conclusion：The extraction technology was economic,feasible and controllable.

关 键 词：广枣 总黄酮 没食子酸 

分 类 号：R283.6[医药卫生—中药学]