HMME—PDT对大鼠致龋模型中变形链球菌抑制作用的研究  

Inhibitive effect of photodynamic therapy with different parameters on caries development in rats

作　　者：马莹莹[1] 邹朝晖[1] 张蕾[1] 李敏[1] 阴慧娟[1] 

机构地区：[1]天津医科大学口腔医院,300070

出　　处：《国际生物医学工程杂志》2011年第2期98-101,共4页International Journal of Biomedical Engineering

摘　　要：目的 探讨不同参数光动力疗法（PDT）在大鼠口腔龋齿预防中的作用。方法用变形链球菌感染Wistar大鼠口腔，建立龋齿模型。以0．9％生理盐水、0．2％氟化钠为对照组，单纯激光、单纯光敏剂、不同参数PDT为实验组。采用血卟啉单甲醚（HMME）为光敏剂，532nm半导体激光为光源，每周处理牙齿1次，5周后处死大鼠，Keyes记分比较龋齿预防效果，扫描电镜观察牙齿表面形态的变化。结果Keyes记分显示：不同参数PDT组、氟化钠组与生理盐水对照组相比Keyes记分较低，其差异有统计学意义（P〈0．05）；单纯激光、单纯光敏剂组、生理盐水组之间Keyes记分差异无统计学意义（P〉0．05）；不同参数PDT组之间Keves记分差异有统计学意义（P〈0．05），并且随着功率的增加，龋齿记分逐渐降低。扫描电镜显示，不同功率的激光照射可造成大鼠牙齿表面形态不同程度的改变。结论HMME-PDT对大鼠龋齿有明显的预防作用，但是其效果和功率关系密切，在今后的临床应用中须寻找合适的功率。Objective To evaluate the inhibitive effect of Photodynamic therapy （PDT） with different parameters on dental caries formation in rats. Methods Wistar rats were infected with S. mutans to establish the rat caries mode 1. The rat＇s teeth were treated with 0.9% NS and 0.2% NaF as controls; laser, photosensitizer and laser plus photosensitizer, respectively as treated groups. Hematoporyrin monomethyl ether（HMME） was used as photosensitizer and laser as the light source. The rats were sacrificed after five weeks. Keyes count was used to measure the inhibitory effect of PDT on dental caries. The surface morphology of the teeth was evaluated by scan electronic microscopy（SEM）. Results The PDT-treated group and NaF-treated group showed significantly reduced Keyes scores compared with the negative controls （P〈0.05）. The laser-treated group, photosensitizer-treated group and NS-treated group showed no significantly reduced Keyes scores compared with the negative controls （P〈 0.05）. The differences of Keyes scores between various parameter PDT treated groups were significant （P〈0.05）. Additionally, Keyes scores reduced gradually with the increasing of laser power. The outcome of SEM showed that the teeth treated with different laser powers exhibited different changes in morphology. Conclusion HMME- PDT had significant inhibitive efficacy on caries development in rats and its inhibitory effect presented close correlation with the power of laser, which indicates that it is important to apply appropriate power in clinical treatment.

关 键 词：光动力疗法 血卟啉单甲醚 致龋模型 

分 类 号：R78[医药卫生—口腔医学] R-332[医药卫生—临床医学]