CD147、基质金属蛋白酶-9及其抑制物-1在不同级别胶质瘤中的表达及相关性研究  

Expressions and correlation of CD147, MMP-9 and TIMP-1 in gliomas of different grade

作　　者：居红格[1] 沈淑萍[1] 耿虹[1] 谢立平[1] 郭建国[1] 蒋剑英[1] 张伟[1] 王丽珍[1] 王欣欣[1] 

机构地区：[1]内蒙古科技大学包头医学院第一附属医院病理科,包头014010

出　　处：《中国综合临床》2011年第5期461-464,共4页Clinical Medicine of China

基　　金：包头市科技计划项目（2009S1001-30）

摘　　要：目的探讨CD147、基质金属蛋白酶（MMP）-9和基质金属蛋白酶抑制物（TIMP）-1在胶质瘤中的表达及其相关性。方法采用免疫组织化学法对78例石蜡包埋的胶质瘤组织和12例正常脑组织进行标记和分析，应用实时荧光定量RT—PCR方法检测CD147mRNA的相对含量。结果CD147、MMP-9、TIMP-1的阳性率分别为62％（48／78）、71％（55／78）、59％（46／78），采用Spearman进行相关性分析，其与胶质瘤的恶性程度均呈正相关（t=0．2671～0．5631，P〈0．01），CD147与MMP-9呈正相关（rs=0．3576，P〈0．01）；CD147、MMP-9、TIMP-1在胶质瘤的临床分级中的表达（低级别组分别为47％、56％和49％；高级别组为80％、89％和71％）差异有统计学意义（×。值分别为9．510、10．702、4．138，P均〈0．05）；在胶质瘤的病理分级中，CD147mRNA相对含量分别为Ⅰ级0．15，Ⅱ级0．27，Ⅲ级0．46，Ⅳ级0．78，随着胶质瘤的级别越高，CD147mRNA相对含量越高。结论CD147、MMP19和TIMP-1可以作为临床判断胶质瘤预后的分子生物学标志。Objective To investigate the expressions of CD147, MMP-9 and TIMP-1 in human gliomas and analyze the correlations. Methods Expressions of CD147, MMP-9 and TIMP-1 were assessed in paraffin- embedded specimens collected from 78 gliomas and 12 benign brain lesion tissues by immunohistochemistry. Real time PCR was performed to detect CD147 mRNA expression. Results The positive rates of CD147, MMP-9 and TIMP-1 expression were 62% （48/78）, 71% （55/78）, 59% （46/78） respectively. We found a significant positive correlation between CD147, MMP-9, TIMP-1 expressions and poor gliomas differentiation by Spearman analysis （rs = 0. 2671 -0. 5631, Ps 〈 0. 01 ）. There was also a significant positive correlation between CD147 and MMP-9 expression （ rs = 0. 3576, P 〈 0. 01 ） . In addition, the expressions of CD147 （47% vs. 80% , X^2 = 9. 510） , MMP-9 （ 56% vs. 89% , X^2 = 10. 702 ） , and TIMP-1 （ 49% vs. 71% , X^2 = 4. 138 ） were significantly higher in advanced gilomas than early gliomas （Ps 〈0. 05 ）. The relative expression levels of CD147 mRNA in gliomas of I to IV pathological grades were 0. 15,0. 27,0. 46,0.78 respectively. Conclusion The expressions of CD147, MMP-9 and TIMP-1 were important characteristic of gliomas, which may serve as biomarkers in the glioma prognostic prediction.

关 键 词：CD147 基质金属蛋白酶-9 金属蛋白酶抑制物-1 胶质瘤 免疫组化 实时荧光定量RT—PCR 

分 类 号：R739.4[医药卫生—肿瘤]