孕期吸入异氟醚对子代大鼠海马神经元突触可塑性的影响  

Effects of isoflurane inhalation during gestation period on plasticity of hippocampal synapses in offspring rats

作　　者：楼艾飞[1] 谭华[1] 鲁惠顺[1] 徐麟皓[2] 周亮[1] 张晓明[2] 

机构地区：[1]浙江大学医学院附属妇产科医院麻醉科,杭州市310006 [2]浙江大学医学院解剖学系,杭州市310006

出　　处：《中华麻醉学杂志》2011年第2期205-207,共3页Chinese Journal of Anesthesiology

基　　金：浙江省医学会临床科研课题资助（491050-W00853）

摘　　要：目的 探讨孕期吸入异氟醚对子代大鼠海马神经元突触可塑性的影响.方法 孕14 d的SD大鼠10只,体重220～250 g,采用随机数字表法,将孕鼠随机分为对照组和异氟醚组,每组5只.对照组大鼠每天单纯机械通气2 h,异氟醚组大鼠每天吸入1.3%异氟醚2 h,至大鼠分娩.子代大鼠出生后4周,采用Morris水迷宫实验测定认知功能,测定结束后处死子代大鼠取脑,分离海马,采用透射电镜观察海马CA1区神经元突触的超微结构,计数海马神经元突触数量,测定突触后致密物质厚度.结果 与对照组相比,异氟醚组子代大鼠逃避潜伏期延长,穿越平台次数减少,海马神经元突触数量减少,突触后致密物质厚度降低（P〈0.05）.结论 孕期吸入异氟醚可通过抑制子代大鼠海马神经元突触的可塑性而降低其认知功能.Objective To investigate the effect of isoflurane inhalation during gestation period on plasticity of hippo-campal synapses in offspring rats. Methods Ten healthy pregnant SD rats at 14 day gestation were randomly divided into 2 gorups （ n = 5 each）： control group （group C） and isoflurane group （group I）. The rats in group C were mechanically ventilated with O2 while in group I the rats inhaled 1.3% isoflurane in O2 for 2 h a day until labor. Four weeks after birth 4 offspring rats from each pregnant rats （2 male, 2 female） were tested for learning and memory abilities using Morris water maze. Then the offspring rats were sacrificed and hippocampi isolated. The synaptic structure of hippocampal CA1 area was examined by trans-electron microscopy. Results Morris water maze test showed that the escape latency was significantly shorter and the number of times of spanning flat roof greater in group C than in group I. The structure of hippocampus was intact in group C but incomplete in group I. Meanwhile the thickness of synaptic density was significantly decreased in group I. Conclusion Isoflurane anesthesia of pregnant rats may induce learning and memory disabilities in offspring rats by inhibiting the plasticity of synaptic structure in hippocampus.

关 键 词：妊娠 异氟醚 海马 突触 神经元可塑性 

分 类 号：R965[医药卫生—药理学]