复方五味子素B逆转多药耐药性人结肠癌细胞THC-8307  

Reversal Effect of γ-Schisandrin Compound on Multidrug Resistance of Human Carcinoma of Colon Cell THC-8307

作　　者：金秀东[1] 徐秋玲[1] 郑学芝[1] 张绪东[1] 刘桂莲[1] 孙卫[1] 

机构地区：[1]牡丹江医学院生理教研室,牡丹江157011

出　　处：《医学综述》2011年第10期1552-1554,共3页Medical Recapitulate

基　　金：黑龙江省自然科学基金(D200813)

摘　　要：目的探讨复方五味子素B(γSC)逆转人结肠癌细胞THC-8307/OXA多药耐药的作用机制。方法采用四甲基偶氮唑蓝(MTT)法测定药物对细胞的毒性作用;通过Western blot法分析药物对THC-8307细胞P糖蛋白(P-gp)多药耐药相关蛋白表达的影响。结果γSC在浓度<12.5 mg/L时对THC-8307和THC-8307/OXA无毒性作用,12.5 mg/L的γSC可明显逆转THC-8307/OXA耐药细胞的耐药性。THC-8307细胞不表达P-gp,THC-8307/OXA细胞P-gp高表达。加入γSC后THC-8307/OXA细胞P-gp蛋白的表达明显下降。结论γSC具有逆转THC-8307/OXA的作用,主要通过下调肿瘤多药耐药细胞内P-gp的表达逆转耐药。Objective To investigate the reversal effect and mechanism of γ-Schisandrin compound（γSC）against multidrug resistance（MDR）of human carcinoma of colon cell THC-8307/OXA.Methods The cytotoxic effect of the therapeutic agents on cells was determined by MTT assay.The expression of P-gp in THC-8307 cells was examined by Westernblot method.Results γSC was nontoxic to THC-8307 cells and THC-8307/OXA when its concentration was less than 12.5 mg/L.However,it obviously reversed the multidrug resistance of THC-8307/OXA.P-gp was highly expressed in THC-8307/OXA while no expression in THC-8307.γSC could decrease the expression of P-gp in THC-8307/OXA.Conclusion γSC reversed the multidrug resistance of tumor THC-8307/OXA cells by the way of decreasing the expression of P-gp.

关 键 词：复方五味子素B 多药耐药 P糖蛋白 

分 类 号：R735.3[医药卫生—肿瘤]