毒黄素的毒性与活性氧的关系  

RELATIONSHIP BETWEEN TOXOFLAVIN AND ACTIVE OXYGEN

作　　者：顾继杰[1] 郑荣梁[1] 赵乃昕[2] 

机构地区：[1]兰州大学生物系 [2]潍坊医学院微生物教研室

出　　处：《潍坊医学院学报》1990年第3期7-10,共4页Acta Academiae Medicinae Weifang

摘　　要：以三株白血病细胞培养与毒黄素作用,用~3H胸苷参与法测定毒黄素的生长抑制效应。对L7712细胞,0.1μg/ml毒黄素可达67.2±8.4％的生长抑制。抑制作用维持18h此后减弱。未见毒黄素使此细胞发生脂质过氧化作用。试验了各种化合物对毒黄素抑制细胞生长的影响。发现触酶,超氧化物歧化酶,甘露醇,二甲亚砜对毒黄素的细胞毒作用没有影响。ATP和丙酮酸盐可部分缓解毒黄素的生长抑制作用。还原性谷胱甘肽则明显缓解毒黄素的细胞生长抑制作用。对毒黄素的素性机理提出一些见解。Growth inhibition effect of toxoflavin on cultures of three lines of leukemia cells has been investigated by ~3H-thymidine incorporation method. In the culture of L7712 cell, toxoflavin in a final concentration of 0.1μg/ml reaches growth inhibition of 67.2±8.4%. The inhibition ef fect by toxoflavin maintains a period of 18h, thereafter declines. No lipid peroxidation has been observed in the system. Various antioxidants have been tested for protecting the cell from the growth inhibition of toxoflavin. It has been found that CAT, SOD, mannitol and DMSO have no influence on the growth inhibition by toxoflavin. ATP and pyruvate can partly restore the growth of the cells. GSH can evidently restore the growth of the cells. By these observations, the mech anism of toxication of toxoflavin has been discussed.

关 键 词：毒黄素 活性氧 还原性 谷胱甘肽 

分 类 号：R595.702[医药卫生—内科学]