羊毛角蛋白/NOCC牙周引导组织再生膜的制备及性能表征  

Preparation and Characterization of Wool Keratin/NOCC Guided Tissue Regeneration Membrane

作　　者：张华林[1] 陈治清[2] 

机构地区：[1]宁夏医科大学口腔医学院,宁夏银川750004 [2]四川大学华西口腔医学院,四川成都610041

出　　处：《哈尔滨医药》2011年第2期81-83,共3页Harbin Medical Journal

基　　金：宁夏医科大学特殊人才科研启动项目(XT201006)

摘　　要：目的制备一种新型的牙周引导组织再生膜并对其性能进行表征。方法先通过还原法制得羊毛角蛋白溶液,再将其与羧甲基壳聚糖(N,O-carboxym ethgl ch itosan,NOCC)复合,采用浇铸成膜法制备羊毛角蛋白/NOCC复合膜;采用XRD、FTIR等检测手段分析复合膜的理化性能和生物相容性。结果羊毛角蛋白/NOCC复合膜表面光滑,结构均一;羊毛角蛋白与NOCC两者分子间存在较强的相互作用,这种相互作用导致了复合膜结晶状态的改变和两组分的相容;复合膜泡胀比和摄水率的平均值分别为6.034和85.75%,拉伸强度、断裂伸长率和杨氏模量的平均值分别为2.53 MPa、45.73%和93.99MPa。结论通过浇铸成膜法制得的羊毛角蛋白/NOCC复合膜具有良好的亲水性、力学性能和生物相容性,满足牙周引导组织再生膜的要求,有望在组织工程领域发挥作用。Objective To prepare a new type of periodontal guided tissue regeneration membrane and characterize its properties.Methods Wool keratin solution was prepared by reduction method,then wool keratin / NOCC composite membranes were prepared by casting film method;using XRD,FTIR and cell culture detection methods to observe the physical and chemical properties,and biocompatibility of the composite membranes.Results The surface of wool keratin/NOCC composite membranes were smooth and uniform;wool keratin and NOCC had strong interactions between molecules,and this interaction lead to changes in crystalline state and the compatibility of the two components;the average value of SR and WU of the composite membranes were 6.034 and 85.75%,respectively;the average value of tensile strength,breaking elongation and Young＇s modulus were 2.53MPa,45.73% and 93.99MPa,respectively.Conclusion The wool keratin / NOCC composite membranes have good hydrophilicity,mechanical properties and biocompatibility;they met the requirements of ideal guided tissue regeneration membrane and are expected to play a role in tissue engineering.

关 键 词：羊毛角蛋白 NOCC 引导组织再生膜 

分 类 号：R784.1[医药卫生—口腔医学]