不同佐剂及免疫途径对重组NTHi外膜蛋白P6免疫效果影响的研究  

The study on the immunizing efficiencies of distinct adjuvants on recombinant P6 protein through different immunization routes

作　　者：陈祥鸭 王健[1] 王涛[1] 李莉莉[1] 张振龙[1] 

机构地区：[1]北京生物制品研究所第四研究室,100024

出　　处：《中华微生物学和免疫学杂志》2011年第4期371-375,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究几种佐剂及免疫途径对重组NTHiP6蛋白抗原在小鼠体内产生的免疫效果的影响。方法以A1（OH）、佐剂、CpGODN佐剂以及两种联合的复合佐荆分别与rP6蛋白混合，经滴鼻、肌肉注射免疫途径免疫6周龄雌性BALB／c小鼠；相间剂量、免疫途径加强免疫2次。采用ELISA法检测rP6蛋白特异的血清IgG和黏膜IgA滴度；酶联免疫斑点试验（ELISPOT）检测特异性T淋巴细胞的活化。结果3次免疫后，CpGODN＋rP6滴鼻免疫组和AI（OH），＋CpGODN＋rP6肌肉注射免疫组产生高滴度的特异性血清IgG抗体（F=41．259，P=0．000）。同时ELISPOT试验显示，CpGODN＋rP6无论通过滴鼻还足肌肉注射免疫均能诱导大量抗原特异性T淋巴细胞的活化，与其他实验组比较差异有统计学意义（F=66．046，P=0．000）。而且CpGODN＋rP6滴鼻免疫组能诱发高滴度的特异性黏膜IgA抗体（F=70．966，P=0．000）。结论重组NTHi外膜蛋白P6与CpGODN佐剂联合后经黏膜途径免疫，小但能保证体液免疫的高滴度血清IgG抗体，而且能从黏膜免疫和细胞免疫两方面对该蛋门的免疫效果进行增强。Objective To detect the difference of cytokines and antibodies productions by immunologic system from mice vaccinated with recombinant P6 protein with different immunization routes and adjuvallls. Methods 6 weeks female BALB/c mice were vaccinated with recombinant protein P6 combined with Al（ OH）3, CpG ODN or the mix of them through intramuscular and intranasal. The mice were boosted twice with the same dose by the same route. Serum and respirato~ tract specimen were collected to detect rP6 specific antibodies by ELISA. Results In the 6th week after immunization, The higher titer of serum rP6 specific IgG antibody were detected from the two groups vaccinated with AI（OH） 3 ＋ CpG ODN ＋ rP6 by intranmscular and vaccinated with CpG ODN ＋ rP6 by intranasal（ F =41. 259, P = 0. 000）. The ELISPOT experiment showed that, Inoculation with the CpG ODN ＋ rP6 either by intranasal or intramuscular immuni- zation could induce a large number of antigen-specific T lymphocyte activation. In addition, The mice vacci- nated with the CpG ODN ＋ rP6 through intranasal can be detected high titer rP6 specific mucosal IgA antibody （ F = 70. 966, P = 0.000 ）. Conclusion Inoculation with the CpG ODN ＋ rP6 by intranasal immunization not only can induce high-titer serum IgG antibody, but also can induce high-titer mucosal IgA antibody and CD＋ Thl, CD8＋ T lymphocyte activation.

关 键 词：P6蛋白 CPG ODN 佐剂 免疫途径 体液免疫 

分 类 号：R512.62[医药卫生—内科学]