β-连环蛋白在KB细胞及其肿瘤球细胞中的表达  

Expression of β-catenin in human KB cell line and tumor sphere cell

作　　者：时伟红[1] 李翀[1] 刘津京[1] 王唯[1] 郑志红[1] 

机构地区：[1]中国医科大学实验动物学部,辽宁沈阳110001

出　　处：《现代肿瘤医学》2011年第5期845-847,共3页Journal of Modern Oncology

基　　金：辽宁省科学技术基金项目(编号:2009408001-3)

摘　　要：目的:研究信号通路β-连环蛋白在人口腔上皮样癌KB细胞及其肿瘤球细胞中的表达。方法:培养KB细胞及其肿瘤球,分别提取KB细胞和肿瘤球细胞的总RNA,设计引物,使用RT-PCR方法检测β-连环蛋白在KB细胞和肿瘤球细胞中的转录,使用Western blot方法检测β-连环蛋白在两种细胞中的翻译。结果:RT-PCR方法检测显示两种细胞的β-连环蛋白的结果均为阳性,KB细胞中β-连环蛋白mRNA含量多于肿瘤球细胞。Western bolt方法检测显示结果均为阳性,KB细胞中β-连环蛋白含量少于肿瘤球细胞。结论:在KB细胞及其肿瘤球细胞中β-连环蛋白转录并积累。Objectivf：To study the expression of signaling pathway β-catenin in human KB cell line and tumor sphere cell.Methods：KB and KB tumor sphere cells were cultured.RNA was extract respectively from KB cell and KB tumor sphere cell.The mRNA of β-catenin was detected by RT-PCR with primer designed by ourselves.The protein of β-catenin was detected by western.Resulsts：β-catenin of two kind cell were positive,but the mRNA amount of KB cell was higher than tumor sphere cell.Western results showes two kind cell were positive,but the protein amount of tumor sphere cell was higher than KB cell.Conclusion： β-catenin protein is accumulated and transcribed in KB cell and KB tumor sphere cell.

关 键 词：KB细胞系 Β-连环蛋白 肿瘤球细胞 口腔癌 

分 类 号：R73-75[医药卫生—肿瘤]