直肠癌组织中hPTTG1和survivin的表达及意义  

The expression and significance of hPTTG1 and survivin in rectal carcinoma tissue

作　　者：殷江霞[1,2] 孙红芹[2] 唐磊[2] 赵长林[2] 徐惠绵[3] 

机构地区：[1]山东省寿光市寿光中医院内三科,山东寿光262700 [2]大连大学附属新华医院,辽宁大连116021 [3]中国医科大学附属第一医院肿瘤科,辽宁沈阳110001

出　　处：《现代肿瘤医学》2011年第5期951-955,共5页Journal of Modern Oncology

摘　　要：目的:检测人垂体瘤转化基因1(human pituitary-tumour transforming gene,hPTTG1)与存活素(sur-vivin)在直肠癌、直肠腺瘤和癌旁正常组织中的表达,分析两者与直肠癌生物学行为的关系和机制。方法:收集2006-2008年间我院直肠癌手术标本60例,直肠腺瘤10例,癌旁正常组织10例,应用免疫组化SP法检测60例直肠癌、10例直肠腺瘤、10例癌旁正常组织中hPTTG1与survivin的表达,分析两者与直肠癌生物学行为的关系及意义。结果:在直肠癌、直肠腺瘤与癌旁正常组织中,hPTTG1的阳性表达率分别为80%(48/60)、20%(2/10)和0(0/10);hPTTG1的表达在直肠癌与直肠腺瘤之间及直肠癌与癌旁正常组织之间差异有显著性意义(P<0.01)。survivin的阳性表达率为90%(54/60)、80%(8/10)和20%(2/10)。survivin的表达在直肠癌和直肠腺瘤组与癌旁正常组织之间差异有显著性意义(P<0.05)。直肠癌中hPTTG1的表达与肿瘤Dukes分期、分化程度,淋巴结转移和分型关系密切(P<0.05)。直肠癌中survivin的表达与肿瘤Dukes分期和淋巴结转移关系密切(P<0.05)。60例直肠癌中两者与患者的性别、年龄、术前CEA、肿瘤所在直肠部位无相关性(均为P>0.05)。结论:hPTTG1和survivin可能在直肠癌的发生发展过程中发挥重要作用,可能与直肠癌侵袭转移密切相关。Objective：To detect the expression of hPTTG1（human pituitary-tumour transforming gene,hPTTG1） and survivin in rectal carcinoma,rectal adenoma and para-cancerous tissue,explore their correlation and mechanism with biological behavior of rectal cancer.Methods： Sixty specimen of rectal carcinoma were collected from January 2006 to December 2008 in our hospital,immunohistochemical SP staining method was used to detect the expression of hPTTG1 and survivin in 60 cases of rectal carcinoma,10 cases of rectal adenoma and 10 cases of para-cancerous tissues,and analyzed the relationship and biological behavior among the speciments.Results： The positive rates of hPTTG1 in rectal carcinoma,rectal adenoma and para-cancerous tissues were 80%（48/60）,20%（2/10） and 0（0/10）,respectively,（P〈0.01）.The positive rates of survivin in rectal carcinoma,rectal adenoma and para-cancerous tissue were 90%（54/60）,80%（8/10） and 20%（2/10）,respectively（P〈0.05）.The expression of hPTTG1 in rectal carcinoma was significantly correlated with Dukes staging,the degree of differentiation,lymph node metastasis and type of the tumor（P〈0.05）.The expression of survivin was significantly correlated with Dukes staging and lymph node metastasis in rectal carcinoma（P〈0.05）.The expression of hPTTG1 and survivn in rectal adenocarcinoma was not correlated with gender and age of patients,CEA,tumor locations of rectum.Conclusion： hPTTG1 and survivn may play an important role in development and progression of rectal carcinoma,show a close correlation with rectal carcinoma invasion and metastasis.

关 键 词：人垂体瘤转化基因1 存活素 直肠癌 侵袭转移 

分 类 号：R735.37[医药卫生—肿瘤]