三氧化二砷与紫杉醇联合诱导Hep-2细胞凋亡的体外研究  

Study in the effect of arsenic trioxide and taxol on Hep-2 in vitro

作　　者：张健梅[1] 文姝[1] 李洁[1] 彭园园[1] 

机构地区：[1]武警北京总队医院耳鼻咽喉头颈外科,北京100027

出　　处：《中国耳鼻咽喉头颈外科》2011年第4期196-199,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的研究三氧化二砷(arsenic trioxide,As2O3)与紫杉醇联合应用对人喉鳞状细胞癌Hep-2细胞株(简称Hep-2细胞)作用的影响及机制。方法以不同浓度的As2O3与浓度为10 nmol/L紫杉醇共同作用于Hep-2细胞,利用瑞氏-吉姆萨(Wrighs-Gimesa)染色及流式细胞术,观察两者对细胞凋亡及细胞周期的影响。结果 As2O3对Hep-2细胞的凋亡率具有药物浓度依赖效应,与未用药组相比,单用10 nmol/L紫杉醇对Hep-2细胞的凋亡率无明显影响。与单用As2O3组相比较,不同浓度As2O3与10 nmol/L紫杉醇具有显著协同作用,其差异有统计学意义(P﹤0.05)。结论 As2O3与紫杉醇联合用药对Hep-2细胞具有诱导凋亡的协同作用。OBJECTIVE To investigate the effect and related mechanisms of arsenic trioxide and Taxol on cell apoptosis of a human laryngeal squamous cell carcinoma cell line（Hep-2）.METHODS Hep-2 cell was treated with arsenic trioxide at different concentrations and 10 nmol/L Taxol,respectively.The apoptosis rate and the cell cycle were evaluated with Wrigh＇s-Gimesa and Flow cytometry.RESULTS The effect of As2O3 on apoptosis rate was concentration dependence.Compared with untreated control group,10 nmol/L Taxol was no signiflcant effect on apoptosis rate of Hep-2 cell.Compared with single As2O3 group,combination of different concentration of As2O3 and 10nmol/L Taxol has synergistic effect.The difference was statistically significant（P0.05）.CONCLUSION Combination of arsenic trioxide and Taxol can induce apoptosis of Hep-2 cell lines.

关 键 词：癌 鳞状细胞 细胞凋亡 抗肿瘤药 红豆杉属 三氧化二砷 

分 类 号：R739.65[医药卫生—肿瘤]