新疆伊犁地区无形体流行病学调查及病原16SrRNA序列分析  

Divergence of Anaplasma isolates from domestic animals in Yili region of Xinjiang Province,China

作　　者：范德生 禹惠兰[2] 吴保新 王誓闻[2] 徐琪毅 张立霞[2] 张丽娟[2] 

机构地区：[1]伊犁州疾病预防控制中心,伊宁835000 [2]中国疾病预防控制中心传染病预防控制所,北京102206 [3]新疆伊犁霍城县疾控中心

出　　处：《中国人兽共患病学报》2011年第4期327-330,共4页Chinese Journal of Zoonoses

基　　金：传染病防治科技重大专项-细菌性传染病病原谱流行规律与变异研究(2009ZX10004-203);病原体实验室网络化监测技术研究(2008ZX10004-008);国家基础研究项目-973计划2010CB530200(2010CB530206)等资助

摘　　要：目的调查新疆地区人、家畜动物包括羊、牛、马及野生动物旱獭、大尾黄鼠及家养马鹿等无形体感染及病原16SrRNA序列特征。方法采集伊犁地区正常人、牛、马、羊及野生啮齿动物大尾黄鼠、旱獭血液。微量间接免疫荧光检测方法(mIFA)检测血清无形体IgMI、gG抗体。巢氏PCR扩增无形体16SrRNA基因并分析序列特征。结果当地人群无形体IgG抗体阳性率为5.3%;羊、牛及马为6.9%、6.3%及9.1%。PCR结果发现羊、牛及马无形体16SrRNA基因检出率为38.9%、37.5%及36.4%。16SrRNA(228bp)序列分析显示存在明显变异株,同源比较结果提示这些变异株在我国其他地区及周边国家媒介及动物宿主中均有广泛分布。结论新疆伊犁地区存在人及家畜无形体感染。开展发热病人无形体实验室调查及进一步人群、媒介种类、宿主与无形体生态学关系研究十分必要。The objective of the present study was to investigate the epidemiological situation of A.phagocytophilum in health population and animals including goats,oxen,horses,marmotas,red deer and Citellus undulatus in Yili regions,Xinjiang Province.Using micro indirect immunofluoresce assay（mIFA）,IgM and IgG antibody against A.phagocytophilum were detected for sera from local healthy people and animals.Nested PCR targeted for 16SrRNA gene of A.phagocytophilum were used to test blood DNA from human and animals.Divergence of sequences of A.phagocytophilum 16SrRNA genes from domestic animals was analyzed.The positive rates of IgG antibody against to A.phagocytophilum in healthy population and goats,oxen and horses were 5.3%,6.9%,6.3% and 9.1% respectively.For PCR test,the potential infection rates of goats,oxen and horses were 38.9%,37.5% and 36.4% respectively.There were significant divergence of A.phogocytophilum isolates in this study and homology analysis showed that they were broadly distributed in other regions of China and other countries around China.Results indicated that infection of A.phagocytophilum in domestic animals and human exited in Yili area of Xinjiang Province.Differential diagnoses of unknown febrile patients should be emphasized in clinics and further investigation of ecological characterization of Anaplasmoses including its vectors and hosts should be practiced.

关 键 词：无形体 流行病学调查 16SRRNA基因 新疆伊犁 

分 类 号：R181.3[医药卫生—流行病学]