金针菇菌种诱变选育的研究  

Research on the Mutation Breeding of Flammulia velutipes

作　　者：陈力力[1] 张倩云[1] 甘文娟[1] 邹正[1] 

机构地区：[1]湖南农业大学食品科学技术学院食品科学与生物技术湖南省重点实验室,湖南长沙410128

出　　处：《生物技术》2011年第2期67-69,共3页Biotechnology

基　　金：湖南农大稳定人才基金项目(09WD34)资助

摘　　要：目的:比较紫外线、微波单因子对金针菇J05Y-c单细胞悬液的诱变效应,并筛选获得优势突变菌株。方法:分别采用不同强度的紫外线、微波单因子对金针菇单细胞悬液进行诱变处理,以出发菌株作为对照,比较诱变致死率、正变率、HC值、菌丝生长量,进行优势菌株筛选、传代稳定性试验。结果:诱变效应的最佳剂量范围在致死率75%～90%的处理组;相同处理时间内,微波诱变的正变率大于紫外诱变的正变率,分别为85.00%和76.67%。采用最大功率700W、脉冲频率2540MHz的微波炉,以中等功率强度处理80s,获得HC值、菌丝生长量比出发菌株提高32.38%、62.16%的优势突变株W8011,连续8次传代遗传性能稳定。结论:在试验的金针菇菌种诱变选育中,微波诱变效应优于紫外线诱变效应。Objective：The mutagenic effects of UV and microwave on Flammulia velutipes J05Y-c were compared,and high mutant would be obtained.Method：Original strain was mutated by UV and microwave;death rate,forward rate,HC,growth rate of mycelium were compared between original and mutant strain;and genetic stability of high mutant was determined.Result： Optimal mutagenic effects were obtained in death rate 75%-90%;the forward rate of microwave treated was higher than that UV treated at the same given time,85.00% and 76.67% respectively;Optimal microwave mutant W8011 was selected by 80s treated with microwave oven 700W 2540MHz,which had increased by 32.38% of HC and 62.16% of growth rate of mycelium over the original strain.Conclusion： microwave was the most potent mutagen.

关 键 词：金针菇 诱变 紫外线 微波 死亡率 正变率 

分 类 号：Q933[生物学—微生物学]