TGF-β1/Smad4信号通路在喉鳞癌中的作用及意义  

Effect and significance of the TGF-β1/Smad4 signaling system in laryngeal squamous cell carcinoma

作　　者：李志章[1] 李钦[2] 耿玉艳 邵阳[1] 

机构地区：[1]泰山医学院研究生部,山东泰安271000 [2]临沂市人民医院耳鼻咽喉-头颈外科,山东临沂276000 [3]沂水县马站人民医院皮肤科,山东沂水276400

出　　处：《山东大学耳鼻喉眼学报》2011年第2期16-18,22,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的探讨转化生长因子β1(TGF-β1)及其信号通路在喉鳞癌发生发展和转移中的作用。方法采用免疫组织化学二步法,检测TGF-β1及其受体Ⅱ(TβRⅡ)和通路下游关键分子Smad4在44例喉鳞癌和10例正常喉黏膜组织中的表达情况,分析其与喉鳞癌发生发展和转移的关系。结果 TGF-β1蛋白在喉鳞癌组的阳性表达率为68.2%,高于正常喉黏膜组30%(P<0.05),且与临床分期及淋巴结转移有关(P<0.05)。TβRⅡ和Smad4蛋白在喉鳞癌组的阳性表达率分别为40.9%、34.1%,在正常喉黏膜组中的阳性表达率分别为80%、80%,差异有统计学意义(P<0.05)。TβRⅡ的阳性表达率与临床分期呈负相关(P<0.05),但与淋巴结转移无关(P>0.05);Smad4表达与组织分化、临床分期及淋巴结转移呈负相关(P<0.05)。结论 Smad4介导的TGF-β1信号通路异常与喉鳞癌的发生发展密切相关,Smad4低表达可促进喉鳞癌的淋巴结转移。Objective To explore the effect and significance of the TGF-β1 signaling system in laryngeal squamous cell carcinoma（LSCC）. Methods The immunohistochemical method was used to detect expression of TGF-β1, TβRII, and Smad4 in 44 cases of LSCC and 10 cases of normal laryngeal mucosa. Correlation between the results and LSCC progress and metastasis was analyzed. Results The expression rate of TGF-β1in the LSCC group was 68.2%, which was significantly different from the normal laryngeal mucosa group（30.0%, P 〈0.05）, and the expression of TGF-β1 was associated with clinical stages and lymph node metastasis （ P 〈 0.05 ）. The positive expression rate of TβRII and Smad-4 was 40.9%, 34. 1% in the LSCC group and 80.0%, 80.0% in the normal laryngeal mucosa group （P 〈 0.05 ）. There was a negative correlation between TβRII and clinical stages （ P 〈 0.05 ）, but no correlation between TβRII and lymph node metastasis（ P 〉 0.05 ）. There was a negative correlation between Smad4 and the clinical index （ P 〈 0.05 ）, including pathological differentiation, clinical stage and lymph node metastasis. Conclusion There is a significant correlation between the abnormal TGF-β1/Smad4 signaling system and LSCC progress. Low expression of Smad4 may accelerate lymph node metastasis of LSCC.

关 键 词：喉肿瘤 癌 鳞状细胞 转化生长因子Β1 转化生长因子受体II SMAD4 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]