共刺激分子OX40/OX40L在小鼠变应性鼻炎发病机制中的作用  

Effect of the costimulatory pathway OX40 /OX40L on pathogenesis of allergic rhinitis in mice

作　　者：李伟[1] 甘敏[1] 刘尚耀[1] 朱凤华[1] 杜明[1] 赵建东[2] 

机构地区：[1]滕州市中心人民医院耳鼻咽喉科,山东滕州277500 [2]山东大学第二医院耳鼻咽喉科,济南250033

出　　处：《山东大学耳鼻喉眼学报》2011年第2期45-47,共3页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的探讨OX40/OX40L在小鼠变应性鼻炎发病机制中的可能作用。方法精选30只清洁级小白鼠,随机分成对照组、模型组、干预组。通过免疫组织化学方法测定对照组和模型组小鼠鼻黏膜中OX40的表达,同时应用ILISA法测定各组中IL-4、IL-10、IFN-γ的水平。结果 OX40在变应性鼻炎小鼠鼻黏膜上皮细胞、腺体细胞、血管内皮细胞、CD4+T细胞中均呈现高表达,OX40主要表达于细胞膜、细胞浆,在对照组中极少表达(P<0.05)。ILISA法测定IL-4、IL-10、IFN-γ在对照组与模型组间差异有统计学意义(P<0.05),模型组和干预组相比差异有统计学意义(P<0.05)。结论 OX40/OX40L在变应性鼻炎小鼠发病机制中起重要作用。Objective To evaluate the role of another costimulatory pathway OX40/OX40 ligand（L） in pathogenesis of allergic rhinitis in mice. Methods 30 mice were divided into three groups： the control group, the allergic rhinitis group, and the OX40LmAb interfere group. Immunohistochemistry was used to examine expression of OX40 in the first two groups. IL-4, IL-10 and IFN--γ in the peripheral blood were analyzed by ELISA. Results OX40 showed a strong expression in the epithelium, glandular epithelium cells, vascular endotheliocytes and CD4 ＋ T cells. OX40 was mainly expressed in cytomembrance and cytoplasm, while OX40 was a stained a little in the control group. There was a statistically significant deviation between the allergic rhinitis and control groups （P 〈 0.05 ）. Compared with the control group, the allergic rhinitis group had higher IL-4 and IL-10 levels and lower IFN-γlevel in peripheral blood （P 〈0.05）. Compared with the allergic rhinitis group, the OX40LmAb interfere group had lower IL-4 level and higher IFN-γlevel in peripheral blood（P 〈0.05）. Conclusion OX40/OX40L plays an important role in pathogenesis of allergic rhinitis in mice.

关 键 词：鼻炎 变应性 OX40(CD134) OX40L IL-4 IL-10 IFN-γ 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]