灵芝多糖对长期大强度运动小鼠巨噬细胞活性及功能的影响  

Effects of Ganoderma Lucidum Polysaccharide on Macrophages Activity and Functions in Long-term High Intensity Exercise Mice

作　　者：史亚丽[1] 蔡德华[2] 高兴喜[2] 

机构地区：[1]鲁东大学体育学院,烟台264025 [2]鲁东大学菌物科学与技术研究院

出　　处：《中国运动医学杂志》2011年第4期354-358,共5页Chinese Journal of Sports Medicine

基　　金："十一五"国家科技支撑计划重点项目(2008BADA1BO4);鲁东大学食用菌创新团队建设项目;鲁东大学学科(体育学)建设经费共同资助

摘　　要：目的:研究灵芝多糖(GLP)对长期大强度运动小鼠巨噬细胞活性及功能的影响。方法:实验小鼠50只随机分为盐水+对照组、盐水+运动组、低剂量GLP+运动组、中剂量GLP+运动组、高剂量GLP+运动组,每组10只。3个GLP组每天分别按50 mg/kg/日GLP(低剂量)、100 mg/kg/日GLP(中剂量)、200 mg/kg/日GLP(高剂量)灌胃。盐水+对照组和盐水+运动组每天灌胃等体积生理盐水,共4周。对照组不运动,4个运动组小鼠每天进行1次45 min负重5%游泳训练,每周6次,为期4周。测定4周后各组小鼠巨噬细胞分泌的NO、IL-1β水平和细胞内GSH及吞噬率、吞噬指数。结果:4周运动后盐水+运动组小鼠腹腔巨噬细胞分泌的NO、IL-1β水平显著下降。盐水+运动组小鼠腹腔巨噬细胞吞噬率显著下降。盐水+运动组小鼠腹腔巨噬细胞吞噬指数呈现下降趋势。中剂量GLP+运动组小鼠腹腔巨噬细胞分泌的NO、IL-1β水平显著高于盐水+运动组,高剂量GLP+运动组显著高于盐水+运动组和低剂量GLP+运动组,中、高剂量GLP+运动组小鼠细胞内GSH水平显著低于盐水+运动组和低剂量GLP+运动组,随GLP剂量增加GSH逐渐下降。低、中、高剂量GLP+运动组小鼠腹腔巨噬细胞吞噬率显著高于盐水+运动组,随GLP剂量增加巨噬细胞吞噬率逐渐提高。中剂量GLP+运动组小鼠腹腔巨噬细胞吞噬指数显著高于盐水+运动组和低剂量GLP+运动组。结论:GLP对大强度运动小鼠腹腔巨噬细胞吞噬功能及分泌IL-1β、NO具有明显的促进作用。Objective To investigate the effects of ganoderma lucidum polysaccharide（GLP）on macrophages activity and functions in long-term high intensity exercise mice.Methods 50 experimental mice were randomly divided into 5 groups： saline water ＋ control group（S＋C）,saline water ＋ training group（S＋T）,low dose GLP ＋ training group（LG＋T）,medium dose GLP ＋ training group（MG＋T）,high dose GLP ＋ training group（HG＋T）,10 mice in each group.Different dosage of GLP were supplemented in GLP groups,50mg/kg/day for low dose group,100mg/kg/day for medium dose group and 200mg/kg/dayfor high dose group.S＋C group and S＋T group were treated with same volume of saline water per day,for 4 week.Four weeks load swimming training program with 5% body weight were conducted in training groups,45mins per day,6 days per week.After 4 weeks,the macrophage secreting NO,IL-1β and intracellular GSH,phagocytic percentage,phagocytic index of mice in each group were measured.Results After 4 weeks exercise,the macrophage secreting NO,IL-1β of peritoneal cavity in S＋T group decreased obviously.The macrophage phagocytic percentage of peritoneal cavity in S＋T group decreased obviously.The macrophage phagocytic index in S＋T group showed decrease.Macrophage secreting NO,IL-1β in MG ＋ T was obviously higher than that in S＋T group,Macrophage secreting NO,IL-1β in HG＋T were obviously higher than that in S＋T group and LG＋T group.The GSH in medium and high dose GLP ＋ T groups was obviously less than that in S＋T and LG＋T group,GSH decreased with the increase of GLP dosage.The macrophage phagocytic percentage of peritoneal cavity in low,medium and high dose GLP＋T group were obviously higher than that in S＋T group,the macrophage phagocytic percentage increased with increase of GLP dosage.Macrophage phagocytic index of peritoneal cavity in MG＋T group was obviously higher than that in S＋T and LG＋T group.Conclusion GLP obviously promoted peritoneal macrophage phagocytic function and IL-1β

关 键 词：灵芝多糖 大强度运动 小鼠 巨噬细胞 

分 类 号：R285.5[医药卫生—中药学]