EphA2在缺氧条件下的表达及其对食管癌细胞体外三维培养的影响  

Expression of EphA2 under hypoxia promotes vasculogenic mimicry in esophageal squamous cell carcinoma cells

作　　者：王频[1] 王璐[1] 丁宗励[1] 曾楷峰[1] 金海林[1] 朱宏[1] 施瑞华[1] 

机构地区：[1]南京医科大学第一附属医院消化科,江苏省南京市210029

出　　处：《世界华人消化杂志》2011年第10期996-1000,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30770991;No.30800511~~

摘　　要：目的:探讨在正常氧分压和缺氧条件下,食管癌细胞中上皮细胞激酶A2(EphA2)表达变化及其对体外三维培养的影响.方法:正常氧分压及缺氧条件下培养食管癌Eca109及TE13细胞,RT-PCR及Western blot分别监测细胞中EphA2表达的变化;EphA2m i R NA干扰质粒转染E c a109和T E13细胞后,采用Matrigel体外三维培养观察正常氧分压和缺氧条件下,Eca109及TE13细胞在抑制EphA2表达前后管腔形成数量的变化.结果:缺氧条件下Eca109和TE13细胞中EphA2表达明显增加(P<0.05).Matrigel体外培养中,缺氧条件下其管腔形成数量明显高于正常氧分压下(P<0.05).miRNA有效抑制EphA2表达后,在正常氧分压和缺氧条件下,管腔数量明显下降,但常氧条件下数量减少更为明显(P<0.01).结论:缺氧环境能诱导肿瘤细胞EphA2高表达,并导致体外三维培养模型中管腔数量增加,抑制EphA2表达能够阻断此现象的发生,提示EphA2在缺氧条件下血管生成拟态形成过程中发挥重要作用.AIM：To investigate the effect of expression of Eph receptor tyrosine kinase A2（EphA2）under normoxia and hypoxia on vasculogenic mimicry in esophageal squamous cell carcinoma cells.METHODS：The expression of EphA2 mRNA and protein was measured by RT-PCR and Western blot in two esophageal cancer cell lines Eca109 and TE13 incubated under normoxia and hypoxia.Eca109 and TE13 cells were then transfected with a plasmid harboring small interfering RNA（siRNA）targeting EphA2.Tubular network formation in Eca109 and TE13 cells before and after siRNA transfection was analyzed ,using the three-dimensional Matrigel culture system under normoxia and hypoxia.RESULTS：The expression of EphA2 in Eca109 and TE13 cells was obviously enhanced under hypoxia（P0.05）.The numbers of tubular networks remarkably increased in both Eca109 and TE13 cells under hypoxia（P0.05）.Although the numbers of tubules obviously increased under both hypoxia and normoxia,the increase was more significant under hypoxia（P 0.01）.Tubule-forming ability of cells transfected with a plasmid harboring small interfering RNA targeting EphA2 was significantly reduced.CONCLUSION：Enhanced expression of EphA2 under hypoxia can increase the numbers of tubular networks in esophageal squamous cell carcinoma cells.Tubule-forming ability of cells transfected with a plasmid harboring siRNA targeting EphA2 was significantly inhibited.EphA2 may play an essential role in the formation of vasculogenic mimicry under hypoxia.

关 键 词：食管鳞癌 上皮细胞激酶A2 血管生成拟态 RNA干扰 

分 类 号：R735.1[医药卫生—肿瘤]