非小细胞肺癌患者呼出气冷凝液中p53基因突变检测的研究  

Detection of p53 gene mutation in exhaled breath condensate of patients with non-small cell lung cancer

作　　者：陈金亮[1] 陈建荣[1] 钱春花[1] 蔡映云[4] 陶国华[2] 周峰[2] 陶一江[1] 陆晨希[3] 

机构地区：[1]南通大学第二附属医院呼吸科,226001 [2]南通大学第二附属医院医学检验中心,226001 [3]南通大学第二附属医院胸外科,226001 [4]复旦大学附属中山医院呼吸病研究所,上海200032

出　　处：《国际呼吸杂志》2011年第10期736-739,共4页International Journal of Respiration

基　　金：基金项目：江苏省＂六大人才高峰＂资助课题（06-B-18）;南通市科委社会发展计划项目（S2006048）

摘　　要：目的 探讨非小细胞肺癌（NSCLC）患者呼出气冷凝液（EBC）中p53基因突变检测的临床意义.方法 采用PCR结合DNA测序法,检测53例NSCLC患者（治疗前）EBC中p53基因第5、6、7、8外显子的突变情况,32名健康体检者EBC标本作为对照.结果 肺癌组（治疗前）EBC标本中扩增到p53基因26例,其中10例检测到p53基因突变,突变率为38.5%;正常对照组EBC标本中扩增到p53基因15名,均未检测到p53基因突变;肺癌患者p53基因突变率高于正常对照组（P＜0.01）.结论 本研究成功扩增并分析了EBC中p53基因变异,将有助于肺癌发病机制研究和临床诊断.Objective To investigate the clinical significance of p53 gene mutation in exhaled breath condensate （EBC） of patients with non-small cell lung cancer （NSCLC）. Methods The mutations of exons 5,6,7 and 8 of p53 gene in EBC of 53 patients with NSCLC and 32 healthy persons were detected hy polymerase chain reaction and DNA sequencing method. Results In NSCLC group,p53 gene of 26 cases was amplified,p53 gene mutation was found in ten cases of them,the mutation rate was 38. 5%. In control group,p53 gene of 15 cases was amplified,p53 gene mutation was not found. The mutation rate of p53 gene in NSCLC group was higher than that in control group （ P 〈 0. 01）. Conclusions This research had successfully amplificated and analyzed p53 gene mutation in EBC,which will be helpful for pathogenesis research and clinical diagnosis of lung cancer.

关 键 词：呼出气冷凝液 非小细胞肺癌 P53基因 

分 类 号：R734.2[医药卫生—肿瘤]