2种逼尿肌功能障碍模型的建立  

Two kinds of Non-invasive Detrusor Dysfunction in Rabbits Models for Acupuncture Mechanism Study

作　　者：韩静[1] 叶笑然[1] 孟宪军[2] 陈玄[1] 黄晓卿[1] 

机构地区：[1]福建省中医药研究院,福建福州350003 [2]厦门大学医学院中医系,福建厦门361005

出　　处：《福建中医药大学学报》2011年第3期22-25,共4页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省自然科学基金资助课题(2009J05070);福建省卫生厅中医药科研重点课题(WZZQ0902)

摘　　要：目的建立满足针刺疗效机理研究的逼尿肌功能障碍模型。方法将新西兰兔随机分为对照组、模型A组、模型B组,3组经尿道插管行膀胱持续以20 mL/h匀速定量灌注并动态测定膀胱内压,模型A组注射0.005%新斯的明10 mL,模型B组注射0.05%山莨菪碱10 mL,对照组注射10 mL生理盐水,记录该期间膀胱内压波动及给药结束即时的尿液排出速度。结果模型A组膀胱内压不稳定波型的例数显著高于对照组,排尿速度显著大于对照组;模型B组稳定型膀胱内压波型的例数显著多于对照组,排尿速度显著小于对照组。结论经静脉匀速注射新斯的明可建立膀胱逼尿肌过度活跃模型,经静脉匀速注射山莨菪碱可建立膀胱逼尿肌过度抑制模型,2种模型均为无创性,为在整体功能完整条件下,对排尿功能障碍性疾病的针刺疗效机理研究提供了动物模型基础。Objective To establish the non-invasive detrusor dysfunction models used for the acupuncture mechanism study. Methods New Zealand rabbits were divided into control group, model group A and model group B. Urinary bladder was quantitatively perfused with uniform speed via transurethral catheterization and cystometry were performed. Rabbits in model group A were given neostigmine （0.005%, 10 mL） by intravenous injection, in model group B and control group were given anisodamine （0.05%, 10 mL）, normal sodium （10 mL） respectively by intravenous injection, and the change of intravesical pressure （IP） was recorded through continuous cystometry and urination flow velocity was recorded at the end of administration. Results In model group A, the number of unstable IP wave was significantly more than that of control group, and the urination flow velocity was significantly faster than that of control group. In model group B, the number of stable IP wave was significantly more than that of control group, and the urination flow velocity was significantly slower than that of control group. Conclusion Intravenous infusion of neostigmine can induce detmsor of bladder hyperactivity, while intravenous infusion of anisodamine can induce detrusor of bladder overinhibition. The two kinds of model are both non-invasive, and they provide bases for the acupuncture mechanism study of voiding dysfunction under the condition of body function integrity.

关 键 词：针刺疗法 逼尿肌 动物模型 新斯的明 山莨菪碱 

分 类 号：R-332[医药卫生]