甲状腺相关性眼病眼外肌来源成纤维细胞的原代培养和鉴定  

Primary culture and identification of fibroblasts derived from extraocular muscle of thyroid-associated ophthalmopathy

作　　者：陆燕[1] 魏锐利[1] 蔡季平[1] 李由[1] 

机构地区：[1]第二军医大学附属长征医院眼科,上海市200003

出　　处：《眼科新进展》2011年第5期401-403,414,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金面上项目(编号:81070758)~~

摘　　要：目的体外培养和鉴定甲状腺相关性眼病眼外肌来源的成纤维细胞,为进一步研究甲状腺相关性眼病眼外肌纤维化发病机制提供实验条件。方法采用组织块培养法,进行细胞形态观察和免疫细胞化学鉴定。结果甲状腺相关性眼病眼外肌成纤维细胞原代培养成功并稳定传代,细胞呈长梭形;细胞免疫化学鉴定:波形蛋白染色阳性,而角蛋白、结蛋白、S-100染色均阴性,证明为中胚层来源的成纤维细胞。结论组织块贴壁法培养的细胞符合甲状腺相关性眼病眼外肌成纤维细胞的形态学和免疫学特征,可作为研究甲状腺相关性眼病眼外肌纤维化的体外模型,为进一步实验打下基础。Objective To culture the fibroblasts derived from extraocular muscle of thyroid-associated ophthalmopathy patients in vitro,and provide the experimental condition for further research on the pathogenesis of extraocular muscle fibrosis of thyroid-associated ophthalmopathy.Methods Extraocular muscle fibroblasts were primary cultured by tissue explant culture technique and identified by morphological and immunocytochemistry analysis.Results The extraocular muscle fibroblasts were cultured and passaged successfully.The cells showed long spindle appearance,their vimentin staining were positive,but their desmin staining,keratin staining and S-100 staining were all negative by immunocytochemistry analysis,indicating that the cells were mesoderm derived fibroblasts.Conclusions The cells cultured by tissue explant culture technique are consistent with typical extraocular muscle fibroblasts characteristics in morphology and immunocytochemical observation.The extraocular muscle fibroblasts are used as cell model in vitro for further research on the mechanism of extraocular muscle fibrosis of thyroid-associated ophthalmopathy.

关 键 词：眼外肌成纤维细胞 甲状腺相关性眼病 细胞培养 鉴定 

分 类 号：R771.3[医药卫生—眼科]