rhPTH_((1-34))对成骨细胞增殖、CollagenⅠ及Osterix mRNA表达的影响  

Effects of recombinant human parathyroid hormone_(1-34) (rhPTH_(1-34)) on osteoblast proliferation and collagen Ⅰ and Osterix mRNA expression

作　　者：闫宇梅[1] 董进[2] 

机构地区：[1]山西医科大学2008级内分泌科硕士研究生,太原030001 [2]山西医科大学第一医院内分泌科

出　　处：《中国骨质疏松杂志》2011年第4期291-295,共5页Chinese Journal of Osteoporosis

摘　　要：目的观察间歇给予重组人甲状旁腺激素(1-34)[rhPTH(1-34)]对体外成骨细胞增殖、Ⅰ型胶原蛋白(Collagen Ⅰ)及Osterix mRNA表达的影响,初步探讨rhPTH(1-34)对体外成骨细胞的作用机制。方法体外培养新生大鼠成骨细胞,间歇循环给予0、10-11、10-10、10-9、10-8、10-7M rhPTH(1-34)干预,(24 h为一循环,前12h给药),共2次,用MTT法检测细胞的增殖;RT-PCR法半定量测定成骨细胞Collagen Ⅰ、Osterix mRNA的表达。结果显示rhPTH(1-34)可明显促进成骨细胞的增殖(P<0.05),促进成骨细胞Collagen Ⅰ和Osterix mRNA表达(P<0.05),10-9 M增殖、表达最明显,呈剂量依赖关系。结论 rhPTH(1-34)可促进成骨细胞的增殖、分化,可能是通过Collagen Ⅰ和Osterix mRNA表达来调节。Objective To observe the effect of rhPTH1-34 on osteoblast proliferation and the mRNA expression of collagen I and Osterix in vitro, and to explore the mechanism of the effect of rhPTH1-34 on osteoblast in vitro. Methods Cultured osteoblasts of the new-born rat were intervened with 0, 10^-1, 10 ^-10, 10^ -9, 10 ^-8, and 10 ^-7M rhPTH1-34, 2 times at the first 12 h in a 24-hour cycle. Cell proliferation was assessed with MTT colorimetric assay. The expression of collagen I and Osterix mRNA were examined with semi-quantitive RT-PCR. Results rhPTH1-34 sifnificantly induced osteoblast proliferation （P 〈0. 05）, and stimulated the expression of collagen I and Osterix （P 〈 0. 05）. It was most remarkable at 10-9 M concentration with a does-dependent manner. Conclusion rhPTH1-34 promotes the proliferation, differentiation of the osteoblast, probably by increasing the expression of collagen I and Osterix mRNA.

关 键 词：重组人甲状旁腺激素(1-34) 成骨细胞 I型胶原蛋白 OSTERIX 

分 类 号：R738.1[医药卫生—肿瘤]