颅咽管瘤细胞系的初步建立  

The Establishment of Craniopharyngioma Cell Line

作　　者：徐建国[1] 刘亮[1] 饶正西[1] 周良学[1] 李强[1] 司马秀田[1] 刘浩[1] 刘志勇[1] 游潮[1] 

机构地区：[1]四川大学华西医院神经外科,成都610041

出　　处：《四川大学学报（医学版）》2011年第3期417-421,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30872646)资助

摘　　要：目的初步建立颅咽管瘤细胞系,为该肿瘤永生型细胞株的最终建立提供实验基础和理论依据。方法经我院影像学和病理学确诊的颅咽管瘤患者,取得新鲜手术标本共36例。其中釉质型21例,鳞状乳头型15例。进行肿瘤细胞原代培养、纯化、传代、冻存、复苏,采用免疫细胞化学SP法对培养细胞行角蛋白(Ck)7染色鉴定细胞来源。通过细胞计数、MTT实验测定细胞的生长曲线、倍增时间。以不同浓度的生长激素和他莫昔芬处理肿瘤细胞,绘制生长曲线。结果本实验共进行肿瘤细胞原代培养36例,培养成功且能够进行传代培养的共29例,成功率为80.6%。Ck7抗体鉴定证实所培养细胞为鳞状上皮源性细胞,细胞倍增时间为(3±0.35)d。生长激素在100 ng/mL时具有最明显的促肿瘤生长作用,他莫昔芬可抑制肿瘤生长。结论本研究初步建立了颅咽管瘤细胞系,可进行有限地连续传代培养。Objective To establish the craniopharyngioma cell line with primary culture which might provide experiment background and evidence for future eternal tumor cell line eatablishment.Methods Thirty six surgical specimens were collected from patients with craniopharyngiomas definited by iconography and pathology examinations in West China Hospital,Sichuan University,including twenty one adamantine epitheliomas and fifteen squamous papillary tumors.The tumor cell was treated through primary cukture,purification,passage,freezing,resuscitation,and identified by keratin 7 staining through SP method.The growth curve and double time were detected through trypan blue dye cell count and MTT assay.The growth of the tumor cells treated with growth hormone（GH） and Tamoxifen was also observed.Results Thirty six primary cultures were done,29 of which were successful and subculture was achieved in 80.6% of all primary cultures.These cell lines were from squamous epithelium by keratin 7 antibody identification,with three days of double time.Proloferative effect of GH was most prevalent at 100 ng/mL,while tamoxifen suppressed cell growth.Conclusion The finite craniopharyngioma cell lines were obtained through primary culture.

关 键 词：颅咽管瘤 细胞系 原代培养 生长激素 

分 类 号：R739.4[医药卫生—肿瘤]