白介素12A基因多态性与OLP的相关性研究  

Association study of interleukin 12A gene polymorphisms with oral lichen planus

作　　者：江晨艳[1] 姚辉[2] 唐国瑶[2] 

机构地区：[1]上海交通大学医学院附属第九人民医院耳鼻咽喉科,上海200011 [2]上海交通大学医学院附属第九人民医院口腔黏膜科.上海市口腔医学研究所.上海市口腔医学重点实验室,上海200011

出　　处：《临床口腔医学杂志》2011年第5期259-262,共4页Journal of Clinical Stomatology

基　　金：上海市科学技术委员会资助(08DZ2271100);上海市重点学科建设项目资助(S30206)

摘　　要：目的:研究华东地区汉族人群IL-12A基因多态性与口腔扁平苔藓(orallichenplanus,OLP)的相关性。方法:采用TaqMan荧光定量PCR法检测华东地区292例OLP患者及686例正常对照者的IL-12A基因的5个SNP位点(rs3024415,rs2243123,rs583911,rs568408和rs2243143),分析IL-12A基因多态性与OLP的相关性。结果:①华东地区健康人群IL-12A基因3'-UTRrs568408位点AA、GA和GG基因型频率分别是0.7%、12.4%和86.8%,而OLP患者分别是1.1%、18.4%和80.5%,其中GA和AA基因型分布频率显著高于正常对照组(P=0.0427)。②正常对照组IL-12A基因rs583911位点AA、GA和GG基因型频率分别是4.2%、38.6%和57.1%,而OLP患者分别是9.5%、36.5%和54%,其中AA基因型分布频率显著高于对照组(P=0.00805)。③糜烂型OLP组IL-12A-rs568408/A等位基因频率显著高于正常对照组,分别为11.6%和6.94%(OR=1.76,95%CI:1.133-2.732,P=0.011)。结论:华东地区汉族OLP人群IL-12A基因rs568408位点存在多态性变异,可能与OLP的疾病易感性和严重程度有关。Objective：To investigate single nucleotide polymorphisms （SNP） of interleukin 12A gene with oral lichen planus （OLP） in Chinese Hans residing in East and South-east China, and to provide an important genetic basis in exploring the disease susceptibility and diverse clinical manifestation. Method：Two hundred and ninety-two patients with OLP and 686 healthy control subjects were recruited in Chinese Hans residing in East and South-east China. Genomic DNA was ex- tracted and PCR products spanning five SNPs of the IL-12A gene at positions （rs3024415, rs2243123, rs583911, rs568408 and rs2243143） were analyzed. The genotype frequencies were calculated and analyzed. Result：①Polymorphism of rs568408, located at the 3 ′-UTR region was found to have a significant association with OLP （P =0.0427）. ②Polymorphism of rs583911 was also found to have a significant association with OLP （P =0.0085）. ③The allele frequencies of IL-12A- rs568408 / A in the erosive O LP group were much higher than that in the healthy group （OR= 1.76,95 % CI： 1.133-2.732, P =0.011）. Conclusion： The variation of IL-12A rs568408 gene polymorphisms seems to have some influence on the disease susceptibility and association with the severity of OLP in this Chinese cohort residing in East and South-east China.

关 键 词：口腔扁平苔藓 单核苷酸多态性 白介素12A基因 

分 类 号：R781.5[医药卫生—口腔医学]