三维荧光二阶校正法直接快速测定血浆样中川芎嗪和阿魏酸含量  

Simultaneous determination of ligustrazine and ferulic acid in human plasma samples by combining excitation-emission matrix fluorescence with secondorder calibration

作　　者：牛景芳[1] 吴海龙[1] 于永杰[1] 张树荣[1] 李淑芳[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《计算机与应用化学》2011年第5期513-517,共5页Computers and Applied Chemistry

基　　金：国家自然科学基金(20775025);国家科技部973预研项目(2007CB216404);国家教育部长江学者和创新团队发展计划项目资助

摘　　要：本文将三维荧光光谱与二阶校正方法相结合用于人体血浆样中的中药药理活性成分川芎嗪和阿魏酸含量的直接测定。尽管预测样存在血浆内源荧光物质的基体干扰,但由于该方法基于"数学分离"的思路和具有"二阶优势",仍能对荧光光谱严重重叠的血浆样中的目标分析物进行直接同时定量测定,为川芎嗪和阿魏酸在体内的代谢监控及其药效研究提供了一种新思路。获得的预测样中川芎嗪和阿魏酸的平均回收率分别为(95.7±2.2)%和(100.8±2.5)%。该方法简单、快速,结果可靠。A simple and rapid method using excitation-emission matrix fluorescent spectra coupled with second-order calibration method based on alternating trilinear decomposition（ATLD） algorithm was proposed for simultaneous quantitative determination of ligustrazine and ferulic acid in human plasma samples.This methodology avoids of tedious extraction and separation steps and overcomes the problem caused by serious fluorescence signal overlapping between analytes and plasma matrices.When the right component number was chosen,reliable result was obtained： the average recoveries of ligustrazine and ferulic acid in prediction samples were（95.7±2.2）%and（100.8±2.5）%,respectively,which means that a new underlying method for monitoring the metabolism of ligustrazine and ferulic acid in human plasma.

关 键 词：三维荧光光谱 川芎嗪 阿魏酸 血浆样 二阶校正 

分 类 号：TQ015.9[化学工程] TP391.9[自动化与计算机技术—计算机应用技术]