石斛兰SIZ1基因的克隆及序列分析  

CLONING AND SEQUENCE ANALYSIS OF SIZ1 GENE IN DENDROBIUM

作　　者：王亚琴[1,2] 钟开新[1] 阳成伟[2] 梁山[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]华南师范大学生命科学学院广东省植物发育生物工程重点实验室,广东广州510631

出　　处：《华南师范大学学报（自然科学版）》2011年第2期119-123,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(30970216);863资助项目(024-D90661);广东省自然科学基金项目(07006553);广东省科技计划项目(2007A020100001)

摘　　要：首次从石斛兰中克隆到1个类SIZ1基因,命名为DenSIZ1,运用生物信息学软件分析与预测其蛋白的理化性质、结构组成、二级结构、功能结构域与亚细胞定位.结果表明,DenSIZ1可能属于PIAS家族SUMO E3连接酶,包括3个重要的功能结构域SAP、PHD和zf-MIZ,且该蛋白可能定位于细胞核中,与PIAS家族有较高的同源性.A SIZ1-like gene was firstly cloned from Dendrobium cDNA library and named as DenSIZ1.Using the bioinformatics software,the physical and chemical properties,composition,secondary structure,tertiary structure,subcellular localization and functional structure domain were analysized and predicted.These results showed that DenSIZ1 probably belongs to PIAS family member of SUMO E3 ligase and includes three important function domains of SAP,PHD and zf-MIZ.Moreover,it is located in cellular nucleus and has higher homology with PIAS family.

关 键 词：石斛兰 SUMO E3连接酶 基因克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]