姜黄素对人膀胱癌T24细胞增殖和凋亡的影响  

The Study of Curcumin Influence on Proliferation and Apoptosis of Human Bladder Cancer T24 Cells

作　　者：孔涛[1] 杨静哲[1] 代宏亮[1] 王成李[1] 张志杰[1] 李巍[1] 李以良[1] 贾玉森[1] 

机构地区：[1]北京中医药大学东方医院泌尿外科男科,北京100078

出　　处：《中国中医基础医学杂志》2011年第5期527-528,共2页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的:旨在探讨姜黄素对人膀胱癌T24细胞增殖和凋亡的影响。方法:细胞传代培养:培养人膀胱癌T24细胞,施加不同浓度姜黄素,观察对细胞的增殖、凋亡作用;应用四唑盐比色法(MTT法)检测细胞增殖抑制率;流式细胞仪检测细胞凋亡率(碘化丙啶染色)。结果:姜黄素各组较对照组增殖降低,存在剂量-效应关系。姜黄素各组较对照组凋亡升高,存在剂量-效应关系。结论:姜黄素抑制人膀胱癌T24细胞增殖,姜黄素促进人膀胱癌T24细胞凋亡。Objectives：The investigation studied curcumin influence on proliferation and apoptosis of human bladder cancer T24 cells.Methods：T24 cell serial subcultivation：Human bladder cancer T24 cell were educated.And different density were added into above cells and incubated to observe influence on proliferation and apoptosis;Cell proliferation inhibition rates were detected by MTT;Cell apoptosis rates were detected by flow cytometer（stained by propidium iodide）.Results：Changes of proliferation morphology and proliferation rates of T24 cells：The condition of proliferation in curcumin groups decreased compared with control group;there were dose-effect（10-500μmol/L）.Changes of apoptosis with T24 cells：The condition of apoptosis in curcumin groups（100,500μmol/L） increased compared with control group;there were dose-effectrelation in diferent curcumin groups.Conclusions：Curcumin can reduce human bladder cancer T24 cells proliferation.Curcumin can accelerate human bladder cancer T24 cells apoptosis.

关 键 词：姜黄素 膀胱癌 T24细胞 增殖 凋亡 

分 类 号：R737.14[医药卫生—肿瘤]