TCT标本检测高危HPV E6/E7mRNA及在宫颈病变中的应用研究  

Practicability Study of Applying High-Risk Human Papilloma Virus E6/E7 mRNA Levels to Determine Cervical Lesion by Using ThinPrep Cytology Test Sample

作　　者：刘桐宇[1] 谢榕[1] Lulu Zhang 郑雄伟[1] 陈刚[2] 何诚[2] 林玉珍[1] 杨琳[1] 李桑[1] 

机构地区：[1]福建省肿瘤医院妇科,福建福州350014 [2]福建省肿瘤医院病理科,福建福州350014 [3]美国纽约罗切斯特大学肿瘤放射中心

出　　处：《中华妇幼临床医学杂志（电子版）》2011年第3期166-169,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：福建省卫生厅青年基金资助项目(2009-2-29)~~

摘　　要：目的探讨新柏超薄液基细胞学检测(Thin-prep cytology test,TCT)标本检测高危人乳头瘤病毒(human papilloma virus,HPV)E6/E7mRNA可行性及在宫颈癌筛查中的应用价值。方法 两种标本处理方法,分别在22例经超薄液基细胞学技术标本检测HPV E6/E7mRNA及HPV E6/E7DNA;对108例不同病变程度宫颈癌标本采取超薄液基细胞学技术检测高危HPV(high-risk HPV,HR-HPV)E6/E7mRNA及高危HPV E6/E7DNA,并行统计学处理。结果 两种方法处理标本行Willcoxon配对秩和检验比较,差异无显著意义(P>0.05)。随着宫颈病变程度增加,HPV E6/E7mRNA表达率相应增加。新柏液基细胞学技术检测108例标本高危HPV E6/E7mRNA表达的相对拷贝数为320.554±779.104,高危HPV E6/E7DNA为1540.963±3575.255,两者秩相关检验,相关系数为1。结论 超薄液基细胞学检测标本可行高危HPV E6/E7mRNA及HPV E6/E7DNA检测;宫颈癌筛查检测HPV E6/E7mRNA有一定临床意义。Objective To research the feasibility and clinical application value of high-risk human papilloma virus （HR-HPV） E6/E7 mRNA and DNA transcripts in cervical cancer samples by using liquid- based Thin-prep cytology test （TCT）. Methods Both high-risk HPV E6/E7 mRNA and DNA of 22 samples by two different methods of Specimen handling were detected. The data from detection of high risk HPV E6/E7 mRNA and HPV E6/E7 DNA in 108 TCT samples with different cervical lesions were statistically analyzed. Results Willcoxon rank test was used to exam two different methods of Specimen handling （P）0. 05）. The expression level of HPV E6/E7 mRNA transcripts was related to the development of severe epithelial cervical dysplasia. The expression copy number of HPV E6/E7 mRNA transcripts in 108 Thin-prep cytology test was 320. 554 ~ 779. 104. The expression copy number of HPV E6/E7 DNA transcripts in 108 Thin-prep cytology tests was 1540. 963 ~ 3575. 255. The coefficient of Spearman test among HPV DNA and E6/E7 mRNA was 1. Conclusions The residual Thin-prep cytology test samples could be used in the detection of high-risk HPV E6/E7 mRNA and HPV E6/E7 DNA. HPV E6 and E7 mRNA test could be a useful tool for cervical cancer screening.

关 键 词：人乳头瘤病毒 MRNA DNA 新柏超薄液基细胞学检查 E6/E7 

分 类 号：R737.33[医药卫生—肿瘤]