原花青素对宫颈癌HeLa细胞Caspase-3和Survivin基因表达的影响  

Effects of Proanthocyanidins on the Expression of Caspase-3 and Survivin Gene in Cervical Cancer HeLa Cell

作　　者：黄雪坤[1] 尚慧玲[1] 张四友[1] 李光仪[1] 

机构地区：[1]佛山市第一人民医院妇产科,山东佛山528000

出　　处：《中华妇幼临床医学杂志（电子版）》2011年第3期185-188,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

摘　　要：目的探讨原花青素对宫颈癌HeLa细胞Caspase-3和Survivin基因表达的影响。方法 将按照不同终浓度原花青素(0,37.5μmol/L,75.0μmol/L,150.0μmol/L,300.0μmol/L和600.0μmol/L)处理宫颈癌HeLa细胞,分别纳入对照组(空白对照),37.5μmol/L PC组,75.0μmol/L PC组,150.0μmol/L PC组,300.0μmol/L PC组和600.0μmol/L PC组,继续培养24h,收集细胞进行实验。采用RT-PCR检测Caspase-3mRNA和Survivin mRNA表达变化。采取Western blot检测caspase-3和survivin蛋白表达的变化。采用比色法检测caspase-3相对活性。结果 随着原花青素浓度增加,Caspase-3mRNA和caspase-3蛋白表达水平逐渐增加,而Survivin mRNA和survivin蛋白表达水平逐渐减少;随着处理宫颈癌HeLa细胞原花青素浓度升高,caspase-3相对活性逐渐增加,且差异有显著意义(P<0.05),在原花青素为600.0μmol/L时,活性最大。结论 原花青素可剂量依赖性地增加Caspase-3mRNA和caspase-3蛋白表达水平及caspase-3活性,并且可剂量依赖性减少Survivin mRNA和survivin蛋白表达水平。Objective To study the effect of proanthocyanidins （PC） on the expression of Caspase-3 and Survivin gene in cervical cancer HeLa cell. Methods After the HeLa cells were treated with different concentration of proanthocyanidins （0, 37. 5 /μmol/L, 75. 0 μmol/L, 150. 0μmol/L, 300. 0 μmol/L and 600.0 μmol/L）. HeLa cells were divided into control group （black control）, 37. 5 μmol/L PC group, 75.0 μmol/L PC group, 150.0 μmol/L PC group, 300.0μmol/L PC group and 600.0 μmol/L PC group. Cells were collected for experiments after culturing for 24 h. Expression of Caspase-3 mRNA and Survivin mRNA were detected by RT-PCR. Expression of caspase-3 protein and survivin protein were detected by Western blot, and caspase-3 relative activities were determined by color metric assay. Results Expression of Caspase-3 mRNA and caspase-3 protein increased with proanthocyanidins concentration from 0 to 600.0 μmol/L, but those of Survivin mRNA and survivin protein decreased. In addition, the activity of caspase-3 was increased （P〈0.05）, and reaching the peak in 600.0 μmol/L in PC group. Conclusion According to increase of concentration of proanthocyanidins, expression of the Caspase-3 mRNA and its protein, and the caspase-3 relative activities in a dose-dependent manner increased. Otherwise, expression of the survivin mRNA and its protein decreased by increasing of concentration of proanthocyanidins, also in a dose-dependent manner.

关 键 词：原花青素 宫颈癌HELA细胞 CASPASE-3基因 SURVIVIN基因 

分 类 号：R737.33[医药卫生—肿瘤]