添加益生元对急进高原大鼠肠黏膜屏障紧密连接蛋白Occludin的作用研究  

The effect of prebiotics on intestinal mucosal barrier protein occludin in model rats acutely exposed to high altitude

作　　者：段云[1] 张方信[2] 单体栋[2] 邵珂[2] 张盼[2] 

机构地区：[1]兰州大学第二临床医学院,甘肃兰州730000 [2]中国人民解放军兰州军区总医院消化内科,甘肃兰州730050

出　　处：《中国微生态学杂志》2011年第5期390-392,397,共4页Chinese Journal of Microecology

基　　金：全军"十一五"计划课题(06MA089)

摘　　要：目的探讨通过灌胃的方式给予益生元(低聚半乳糖)对急进高原大鼠肠黏膜屏障紧密连接Occludin蛋白的表达及作用。方法在3848米建立高原缺氧模型大鼠共48只,随机选24只为高原缺氧对照组,另24只为添加益生元组,再按2 d、4 d和6 d时间点,分为6组,每组8只。采用免疫组织化学法检测Occludin蛋白表达,用ELISA法测定回肠组织肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)。结果和高原缺氧组各时间点比较,添加益生元组Occludin蛋白表达显著升高(P<0.01),TNF-α水平降低、IL-10水平升高(P<0.05)。结论益生元可以增加急进高原缺氧大鼠肠黏膜紧密连接蛋白Occludin的表达,改善高原缺氧对大鼠肠黏膜屏障的破坏。Objective To investigate the effect of prebiotics on the expression of occludin intestinal mucosal barrier in model rats acutely exposed to high altitude.Method Forty-eight rat models in radical plateau hypoxia environment were established（3 848 m）.These rats were randomly divided into hypoxia control group（n=24） and prebiotics group（n=24）,then further randomly divided into six groups（n=8） by 2 d,4 d and 6 d time points,respectively.The expression of occludin protein in intestinal mucosa was analyzed by immunohistochemistry method,and Tumor necrosis factor-α（TNF-α） and interleukin-10（IL-10） in ileum tissue were determined with ELISA.Result At each time point,occludin protein in all hypoxia control groups were significantly lower than in prebiotics group（P0.01）,while TNF-α was significantly increased and serum IL-10 was remarkably reduced（P0.05）.Conclusion Prebiotics has the effect of maintaining the integrity of epithelial cells by increasing occludin protein expression and reducing the damage of intestinal mucosal barrier caused by hypoxia.

关 键 词：高原 缺氧 益生元 OCCLUDIN蛋白 肠黏膜屏障 

分 类 号：R657.31[医药卫生—外科学]