没食子中没食子鞣质的提取工艺及免疫作用研究  

Extraction of Gallotannins from Galla Turcica and Their Immune Effects

作　　者：范晓红[1] 李治建 斯拉甫.艾白 刘发 周露[1] 孟繁龙[1] 

机构地区：[1]新疆石河子大学药学院,832000 [2]新疆维吾尔自治区维吾尔医药研究所,乌鲁木齐830049 [3]新疆维吾尔自治区维吾尔医医院,乌鲁木齐830049

出　　处：《医药导报》2011年第6期695-698,共4页Herald of Medicine

基　　金：国家科技支撑计划(基金编号:2007BAI30B03);新疆维吾尔自治区科技支疆工程资助项目(基金编号:200840102-34)

摘　　要：目的优选提取没食子鞣质的提取工艺,探讨不同提取物对小鼠免疫功能的影响。方法没食子药材经不同提取方法提取后,各滤液经不同溶剂萃取得鞣质。以鞣质水解后没食子酸提取率为指标,用高效液相色谱(HPLC)法测定含量。采用噻唑蓝(MTT)法检测不同提取工艺的没食子鞣质对小鼠淋巴细胞增殖反应的影响,中性红法检测对小鼠腹腔巨噬细胞吞噬功能的影响。结果甲醇超声提取没食子酸提取率最高,达(77.01±2.97)%;没食子鞣质对小鼠淋巴细胞增殖具有明显的促进作用,可增强小鼠腹腔巨噬细胞吞噬中性红的能力。结论 70%甲醇超声提取为最佳提取方法;没食子鞣质对正常小鼠免疫功能有一定的增强作用。Objective To optimize the extraction process of gallotaunins from Galla Turcica and study their immunological function in vitro. Methods Tannins were extracted from the crude extracts of Calla Turcica with different solvents. Gallic acids were determined by high performance liquid chromatography with hydrolysates of tannins as an indicator. The effects of the gallotannins from Calla Turcica produced by different extraction technology on lymphocyte proliferation were investigated in vitro with MTI＇ method,which on macrophage cells activity were determined by using neutral red assay. Results The content of gallic acid obtained by methyl alcohol supersonic way reached the highest level （77.01±2.97 ） % comparing with other extracting solvents. Gallotannins from Calla Turcica significantly accelerated lymphocytes proliferation and enhanced macrophages phagocytosis of neutral red. Conclusion 70% methyl alcohol supersonic method is the best extraction method for total tannins isolation from Calla Turcica. It demonstrates that gallotannins from Calla Turcica potentiates immune function.

关 键 词：没食子 没食子酸 色谱法 高效液相 淋巴细胞 巨噬细胞 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]