非小细胞肺癌组织中ERCC1基因的表达及临床意义  

Expression of ERCC1 Gene in Non-small Cell Lung Cancer and its Clinical Significance

作　　者：冷雪峰[1] 冼磊[1] 陈铭伍[1] 李满红[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,南宁市530021

出　　处：《广西医学》2011年第5期540-543,共4页Guangxi Medical Journal

基　　金：广西科学研究与技术开发计划项目(10124001A-44);广西研究生创新教育计划项目(2010105981002M181);广西大型仪器网测试资助项目

摘　　要：目的探讨非小细胞肺癌(NSCLC)组织ERCC1基因mRNA表达情况及其临床意义。方法采用反转录-聚合酶链反应(RT-PCR)方法检测82例肺癌组织、36例癌旁组织及15例良性组织中ERCC1基因mRNA的表达情况。结果 ERCC1基因在NSCLC患者癌、癌旁及良性组织中均有表达,分别为54.9%(45/82)、44.4%(16/36)和73.3%(11/15),三者总体差异无统计学意义(P>0.05)。在临床、病理等特征中腺癌表达率63.0%(34/54)高于鳞癌39.3%(11/28),差异有统计学意义(P<0.05)。与其他临床及病理特征均无关联(P>0.05)。结论 ERCC1基因mRNA在不同组织中的表达无显著差异,但其在广西人群中腺癌表达高于鳞癌,可指导患者术后铂类药物的个体化治疗。Objective To detect the expression of excision repair cross complement group 1（ERCC1）gene mRNA in non-small cell lung cancer and study their clinical significance.Methods The expression of ERCC1 gene 82 cases of lung cancer,36 cases of adjacent tissues and 15 cases of benign tissue mRNA were detected by reverse transcription-polymerase chain reaction（RT-PCR）.Results ERCC1 gene in NSCLC patients with carcinoma,adjacent and benign tissues were expressed 54.9%（45/82）,44.4%（16/36）and 73.3%（11/15）,respectively,there was no statistical significance among the three groups（P0.05）.In the clinical and pathological features,63.0% expressed in adenocarcinoma（34/54）were more than that in squamous cell carcinoma 39.3%（11/28）,the difference was statistically significant（P0.05）,and ERCC1 gene did not significantly associated with other clinical and pathological features（P0.05）.Conclusion The expression of ERCC1 gene mRNA in different tissues is not significant,but its expression of adenocarcinoma in the Guangxi population is more than squamous cell carcinoma patients,which may help guide individual platinum drug treatment.

关 键 词：非小细胞肺癌 ERCC1基因 反转录-聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]