反相高效液相色谱三七乳膏中人参皂苷的含量  

Simultaneous Determination of Ginsenoside in Panax Cream by RP-HPLC

作　　者：郭珉[1] 王颖[1] 王栋[1] 张志勤[1] 邵丰德[1] 

机构地区：[1]大连市皮肤病医院药剂科,辽宁大连116021

出　　处：《辽宁中医药大学学报》2011年第6期247-248,共2页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的：建立反相高效液相色谱法,测定三七乳膏中人参皂苷（Rg1）的含量。方法：固定相采用Kromasil-C18柱（150mm×4.6mm ID,5μm）;流动相为：乙腈-水（0.05%磷酸溶液）（60：40,v/v）,流速1.5mL.min-1,检测波长203nm。结果：图谱显示通过改变流速缩短了出峰的保留时间,同时阴性对照也显示了此处不受乳膏基质成分的干扰;人参皂苷（Rg1）在1252000μg.mL-1范围内具有良好的线性关系（r=0.9993）,线性方程为Y=123189x＋200,平均回收率分别为97.9%（n=3）。精密度RSD为0.7%。结论：该方法操作简便,快速,精密度高,专属性好,结果准确,可作为该制剂质量控制标准。Objective：To develop a reversed-phase HPLC method for determination of Rg1 in Radix Notoginseng（san qi）cream.Methods：The Rg1 was chromatographically separated on a Kromasil-C18 column（150mm×4.6mm ID,5μm）using a isocratic mobile phase composed of acetinitrile-0.05% phosphoric acid solution（60：40,v/v）running at a flow rate of 1.5mL·min-1.The detection wavelength was set at 203nm.Results：The HPLC resulted in baseline separation of Rg1 with no interference from cream matrix,good linearity ranged from 125μg·mL-1 to 2000μg·mL-1（r=0.9993）with a linearity equation of Y=123189x＋200,average recovery of 97.9% and precision（RSD）of 0.7%.Conclusion：This method is easy and quick to operate and has high precision,specificity and accuracy,and thus can be used for quality control of this cream preparation.

关 键 词：RG1 HPLC 三七 膏 

分 类 号：R122.11[医药卫生—环境卫生学]