孟加拉红荧光法辅助诊断口腔癌癌前病变的实验研究  

A pilot study of Rose Bengal fluorometric method in auxiliary diagnosis of oral precancerosis

作　　者：张磊[1] 张晓丹[1] 孙祥云[1] 毕良佳[2] 李蕾[1] 袁杰[1] 

机构地区：[1]哈尔滨医科大学附属口腔医学院口腔预防保健科,哈尔滨150001 [2]哈尔滨医科大学附属第四医学院口腔科,哈尔滨150001

出　　处：《口腔医学》2011年第4期203-205,215,共4页Stomatology

基　　金：黑龙江省教育厅科学技术研究项目(11541148)

摘　　要：目的应用动物模型探讨孟加拉红(RB)介导的荧光光谱诊断法辅助诊断各期口腔癌癌前病变的可行性。方法在405 nm的激发光下测定6种不同浓度RB溶液的光谱,并分析荧光强度与浓度之间的关系。20只金黄地鼠随机分成4组(对照组、4周、6周、8周组),每组5只。各组实验动物双侧颊囊经DMBA诱导相应时间后经0.1 g/L RB溶液染色5 min,应用光谱诊断装置分别记录自体及RB激发荧光并应用Origin 7.0软件计算荧光比值K值。检测位点经病理切片定性后计算各组K值均值,SPSS 10.0进行统计分析。结果随着RB溶液浓度的提高,荧光强度亦增强,二者呈正态线性关系。动物实验结果经方差分析,4组K值均值相比有统计学意义(P<0.01)。结论 RB介导的荧光光谱法能够区分正常、单纯增生、轻度异常增生和重度异常增生的组织,对口腔癌癌前病变的诊断具有重要的参考价值。Objective To investigate the feasibility of fluorescence spectra diagnose mediated with Rose Bengal(RB) at different stages of oral precancerous lesions in animal models.Methods Excitated by 405 nm light,fluorescence spectrum of 6 different concentrations of RB solutions were detected under 405 nm light.The relation between fluorescence intensity and concentration was analyzed.20 golden hamsters were randomly divided into 4 groups(control group,4 weeks group,6 weeks group,and 8 weeks group),each group having 5 animals.After inducted by DMBA in corresponding weeks,all the cheek pouches of animal models in each group were dyed by 0.1g/L RB solution for 5 minutes.RB fluorescence and auto-fluorescence spectrums were detected by fluorescence spectroscopy diagnosis system and were analyzed by Origin7.0 to calculate fluorescence ratio K.All the detected points were determined by pathological diagnose.Mean K values were calculated and statistically analyzed by SPSS 10.0.Results The fluorescence intensity of RB enhanced with the increase of concentration.They submitted positive linear relationship.The result of animal experiment was analyzed by ANOVA.It showed that the comparisons of K values in the 4 experiment groups had statistical significance(P<0.01).Conclusions Fluoresscent-spectrofluorometry method mediated by RB could discriminate normaltissue,simple hyperplasia,mild and serious abnormal hyperplasia.It had important reference value in diagnose of oral precancerous lesions.

关 键 词：孟加拉红 光谱 口腔癌 癌前病变 诊断 金黄色地鼠 

分 类 号：R739.85[医药卫生—肿瘤]